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Before LIPSS fabrication, initial sample surface roughness is adjusted by variations of finial polishing steps with polishing grain sizes of 18.3, 8.4, 5, and 0.5 \u00b5m. A 3\u00a0\u00d7 \u00a03 irradiation matrix was\u00a0defined and lasered on all samples by changing the laser power and distance between consecutive laser scans. The resulting structures were\u00a0characterized by scanning electron microscopy (SEM), atomic force microscopy, Raman spectroscopy, and contact angle measurements. As a further step, three representative generated structures were\u00a0chosen to explore their bone implant viability by resazurin assays, alkaline phosphatase activity, and direct SEM imaging of the induced cells (MG63) and bacteria (<jats:italic>Escherichia coli<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic>). Results show that initial surface roughness has big influence on the wettability of the resulting surface, as well as inducing small variations on the orientation of the generated LIPSS. Structures generated with a higher integrated fluence have also shown to enhance cell differentiation while reducing bacterial activity, making them a great candidate for improved bone implant compatibility and durability.<\/jats:p>","DOI":"10.1002\/admt.202201802","type":"journal-article","created":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T10:11:10Z","timestamp":1680689470000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Influence of Initial Surface Roughness on LIPSS Formation and Its Consecutive Impact on Cell\/Bacteria Attachment for TiAl6V4 Surfaces"],"prefix":"10.1002","volume":"8","author":[{"given":"Lamborghini","family":"Sotelo","sequence":"first","affiliation":[{"name":"Institute for Nanotechnology and Correlative Microscopy eV INAM \u00c4u\u00dfere N\u00fcrnberger Str. 62 91301 Forchheim Germany"},{"name":"Friedrich\u2013Alexander University Erlangen\u2013N\u00fcrnberg Staudtstra\u00dfe 7 91058 Erlangen Germany"}]},{"given":"Tommaso","family":"Fontanot","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Ceramic Technologies and Systems IKTS \u00c4u\u00dfere N\u00fcrnberger Str. 62 91301 Forchheim Germany"}]},{"given":"Sanjana","family":"Vig","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Dentaria Universidade do Porto Rua Dr. Manuel Pereira da Silva Porto 4200\u2010393 Portugal"},{"name":"LAQV\/REQUIMTE University of Porto Porto 4160\u2010007 Portugal"}]},{"given":"Patrick","family":"Herre","sequence":"additional","affiliation":[{"name":"Institute for Nanotechnology and Correlative Microscopy eV INAM \u00c4u\u00dfere N\u00fcrnberger Str. 62 91301 Forchheim Germany"}]},{"given":"Peyman","family":"Yousefi","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Ceramic Technologies and Systems IKTS \u00c4u\u00dfere N\u00fcrnberger Str. 62 91301 Forchheim Germany"}]},{"given":"Maria Helena","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Dentaria Universidade do Porto Rua Dr. Manuel Pereira da Silva Porto 4200\u2010393 Portugal"},{"name":"LAQV\/REQUIMTE University of Porto Porto 4160\u2010007 Portugal"}]},{"given":"George","family":"Sarau","sequence":"additional","affiliation":[{"name":"Institute for Nanotechnology and Correlative Microscopy eV INAM \u00c4u\u00dfere N\u00fcrnberger Str. 62 91301 Forchheim Germany"},{"name":"Fraunhofer Institute for Ceramic Technologies and Systems IKTS \u00c4u\u00dfere N\u00fcrnberger Str. 62 91301 Forchheim Germany"},{"name":"Max Planck Institute for the Science of Light Staudtstr. 2 91058 Erlangen Germany"}]},{"given":"Gerd","family":"Leuchs","sequence":"additional","affiliation":[{"name":"Friedrich\u2013Alexander University Erlangen\u2013N\u00fcrnberg Staudtstra\u00dfe 7 91058 Erlangen Germany"},{"name":"Max Planck Institute for the Science of Light Staudtstr. 2 91058 Erlangen Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4908-4087","authenticated-orcid":false,"given":"Silke","family":"Christiansen","sequence":"additional","affiliation":[{"name":"Institute for Nanotechnology and Correlative Microscopy eV INAM \u00c4u\u00dfere N\u00fcrnberger Str. 62 91301 Forchheim Germany"},{"name":"Fraunhofer Institute for Ceramic Technologies and Systems IKTS \u00c4u\u00dfere N\u00fcrnberger Str. 62 91301 Forchheim Germany"},{"name":"Frei Universit\u00e4t Berlin  Arnimalle 14 14195 Berlin Germany"}]}],"member":"311","published-online":{"date-parts":[[2023,4,5]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.1703071"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2351\/1.4712658"},{"key":"e_1_2_10_3_1","first-page":"3","volume":"23","author":"Bonse J.","year":"2016","journal-title":"IEEE J. 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