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Here, a new combination between magneto\u2010responsive anisometric PEG microgels and collagen hydrogels is explored to establish anisotropic in vitro models. Different sizes of PEG microgels are tested to assess the impact of both width and aspect ratio on the formation and alignment of collagen hydrogels. Results show that the key properties of collagen hydrogels, regarding fibrillogenesis, rheological properties, and fiber diameter are kept consistent upon the combination with PEG microgels. Furthermore, partial collagen fiber alignment is observed when larger (width 10\u00a0\u00b5m) PEG microgels are employed and magnetically aligned. In vitro studies show cell alignment within the anisotropic collagen hydrogels from the first day in culture. Interestingly, PEG microgels with higher width and length tend to induce less hydrogel contraction even after 7 days in culture. The results demonstrate the ability to establish a 3D unidirectional collagen hydrogel by magnetically aligning anisometric microgels during the gelation process, which can be promising for different tissue engineering applications.<\/jats:p>","DOI":"10.1002\/admt.202301391","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T06:26:58Z","timestamp":1707978418000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Engineering Anisotropic Cell Models: Development of Collagen Hydrogel Scaffolds with Magneto\u2010Responsive PEG Microgels for Tissue Engineering Applications"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2559-9158","authenticated-orcid":false,"given":"Ana L.","family":"Castro","sequence":"first","affiliation":[{"name":"i3S \u2013 Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Universidade do Porto  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A.","family":"Barbosa","sequence":"additional","affiliation":[{"name":"i3S \u2013 Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Universidade do Porto  4200\u2010135 Porto Portugal"},{"name":"INEB \u2013 Instituto de Engenharia Biom\u00e9dica Universidade do Porto  4200\u2010135 Porto Portugal"},{"name":"ICBAS \u2013 Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar Universidade do Porto  4050\u2010313 Porto Portugal"}]},{"given":"Raquel M.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"i3S \u2013 Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Universidade do Porto  4200\u2010135 Porto Portugal"},{"name":"INEB \u2013 Instituto de Engenharia Biom\u00e9dica Universidade do Porto  4200\u2010135 Porto Portugal"},{"name":"ICBAS \u2013 Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar Universidade do Porto  4050\u2010313 Porto 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