{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T07:54:51Z","timestamp":1761292491403,"version":"build-2065373602"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,1,4]],"date-time":"2008-01-04T00:00:00Z","timestamp":1199404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Adv Synth Catal"],"published-print":{"date-parts":[[2008,1,25]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>2\u2010Oxoadipoyl\u20107\u2010ACA is an intermediate in the conversion of cephalosporin C (CPC) to 7\u2010aminocephalosporanic acid (7\u2010ACA) when using a new route involving <jats:sc>D<\/jats:sc>\u2010amino acid oxidase, catalase and glutaryl acylase. A key point in the reaction design is to avoid the accumulation of hydrogen peroxide in the reaction medium as the yields of 7\u2010ACA decrease in the presence of this compound due to its low stability. Looking for an enzyme with improved activity towards 2\u2010oxoadipoyl\u20107\u2010ACA, different mutants of glutaryl acylase from <jats:italic>Pseudomonas<\/jats:italic> SY\u201077 with an improved activity towards adipoyl\u20107\u2010ACA were evaluated. The best results on 2\u2010oxoadipoyl\u20107\u2010ACA hydrolysis were found with the double mutant Y178F+F375H, which showed a K<jats:sub>cat<\/jats:sub> increase of 6.5\u2010fold and a K<jats:sub>m<\/jats:sub> decrease of 3\u2010fold compared to the wild\u2010type (wt) enzyme. When this enzyme was tested in the tri\u2010enzymatic system to convert CPC into 7\u2010ACA, this mutant permitted us to reach more than an 80\u2009% yield of 7\u2010ACA using a 3\u2010fold mass excess compared to DAAO; while the wt enzyme gave only a 40\u2009% yield. Therefore, the application of this new mutant to the one\u2010pot conversion of CPC to 7\u2010ACA gives very good result in terms of efficiency, yield and rate of the process.<\/jats:p>","DOI":"10.1002\/adsc.200700320","type":"journal-article","created":{"date-parts":[[2008,1,4]],"date-time":"2008-01-04T10:19:30Z","timestamp":1199441970000},"page":"343-348","source":"Crossref","is-referenced-by-count":19,"title":["Evaluation of Different Glutaryl Acylase Mutants to Improve the Hydolysis of Cephalosporin C in the Absence of Hydrogen Peroxide"],"prefix":"10.1002","volume":"350","author":[{"given":"Fernando","family":"L\u00f3pez\u2010Gallego","sequence":"first","affiliation":[]},{"given":"Lorena","family":"Betancor","sequence":"additional","affiliation":[]},{"given":"Charles\u2005F.","family":"Sio","sequence":"additional","affiliation":[]},{"given":"Carlos\u2005R.","family":"Reis","sequence":"additional","affiliation":[]},{"given":"Pol Nadal","family":"Jimenez","sequence":"additional","affiliation":[]},{"given":"Jose\u2005M.","family":"Guisan","sequence":"additional","affiliation":[]},{"given":"Wim.\u2005J.","family":"Quax","sequence":"additional","affiliation":[]},{"given":"Roberto","family":"Fernandez\u2010Lafuente","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2008,1,18]]},"reference":[{"key":"e_1_2_1_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-003-1274-y"},{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/hlca.19680510513"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.12.5821-5826.1987"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.08.110"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.051671705"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260460603"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(94)90139-2"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/0738-859891224194"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(00)00222-2"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02921538"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.200505099"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(98)00028-3"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2004.06.006"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0195-58"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208317200"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2002.03143.x"},{"key":"e_1_2_1_17_2","unstructured":"C.\u2005F. 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