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In response to regulatory efforts to reduce animal testing, there is increasing demand for standardized, scalable, and sensor\u2010integrated microphysiological platforms. Although organ\u2010on\u2010chip technologies have improved biological relevance, many remain limited by technical complexity and insufficient sensing, particularly for non\u2010equilibrium transport processes that characterize barriers such as the renal tubule, intestine, bloodbrain barrier, and placenta. Here, we present a fully automated microfluidic platform for dynamic and quantitative characterization of molecular transport across biomimetic interfaces. The system emulates key biophysical features, including directional flow, controlled gradient formation, and tunable shear stress, using synthetic, cell\u2010free architectures. Integrated oxygen sensors enable monitoring of physiologically relevant oxygen levels, while embedded flow sensors support closedyloop control of fluid dynamics and solute delivery. Together, these sensing capabilities allow experimental control and time\u2010resolved analysis of molecular transport under non\u2010equilibrium conditions. Validated using acellular models, the platform is engineered for future integration with living tissues and molecular sensorization. Proof\u2010of\u2010concept studies demonstrate its ability to reproduce key transport dynamics, highlighting its potential for pharmacokinetic modeling, organ\u2010on\u2010chip research, and preclinical drug development.<\/jats:p>","DOI":"10.1002\/adsr.202500166","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T13:02:18Z","timestamp":1769605338000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Automated Microfluidic Platform for Molecular Transport Analysis across Biomimetic Interfaces"],"prefix":"10.1002","volume":"5","author":[{"given":"Olivier","family":"Fournier","sequence":"first","affiliation":[{"name":"Microfluidics Innovation Center  Paris France"}]},{"given":"Miguel A. D.","family":"Neves","sequence":"additional","affiliation":[{"name":"Institute for Research and Innovation in Health (i3S) University of Porto  Porto Portugal"},{"name":"Molecular and Analytical Medicine Laboratory Department of Biomedicine Faculty of Medicine University of Porto  Porto Portugal"}]},{"given":"Th\u00e9o","family":"Gavoille","sequence":"additional","affiliation":[{"name":"Microfluidics Innovation Center  Paris France"}]},{"given":"Alan","family":"Morin","sequence":"additional","affiliation":[{"name":"Microfluidics Innovation Center  Paris France"}]},{"given":"Beatriz","family":"Pais","sequence":"additional","affiliation":[{"name":"Institute for Research and Innovation in Health (i3S) University of Porto  Porto Portugal"},{"name":"Molecular and Analytical Medicine Laboratory Department of Biomedicine Faculty of Medicine University of Porto  Porto Portugal"},{"name":"NOVA School of Science and Technology Physics Department NOVA University of Lisbon  Caparica Portugal"}]},{"given":"Ivana","family":"Brenta","sequence":"additional","affiliation":[{"name":"Microfluidics Innovation Center  Paris France"}]},{"given":"Justine","family":"Lereculey\u2010Beaumanoir","sequence":"additional","affiliation":[{"name":"Microfluidics Innovation Center  Paris France"}]},{"given":"Andrea","family":"Cruz","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory (INL)  Braga Portugal"}]},{"given":"Denis","family":"Santos","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory (INL)  Braga Portugal"}]},{"given":"Hugo","family":"Oliveira","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory (INL)  Braga Portugal"}]},{"given":"Michael","family":"Gasik","sequence":"additional","affiliation":[{"name":"Seqvera Ltd. Oy  Helsinki Finland"}]},{"given":"Lisa D.","family":"Muiznieks","sequence":"additional","affiliation":[{"name":"Microfluidics Innovation Center  Paris France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8009-7767","authenticated-orcid":false,"given":"In\u00eas","family":"Mendes Pinto","sequence":"additional","affiliation":[{"name":"Institute for Research and Innovation in Health (i3S) University of Porto  Porto Portugal"},{"name":"Molecular and Analytical Medicine Laboratory Department of Biomedicine Faculty of Medicine University of Porto  Porto Portugal"}]}],"member":"311","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41573\u2010019\u201000074\u2010z"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.1166"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3681"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2236"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2989"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2011.09.005"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010020\u20100079\u20103"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201701000"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576\u2010022\u201000466\u20109"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0LC00424C"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204\u2010020\u201002939\u20104"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.0c00574"},{"key":"e_1_2_10_14_1","article-title":"Blood\u2010brain Barrier Transporters: An Overview of Function, Dysfunction in Alzheimer's Disease and Strategies for Treatment","volume":"1870","author":"Chaves J. 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Recommendations on the Conversion Between Oxygen Quantities for Bio\u2010Argo Floats and Other Autonomous Sensor Platforms \u201d (2018) https:\/\/doi.org\/10.13155\/45915."},{"key":"e_1_2_10_39_1","unstructured":"M.GasikandB.Bilotsky \u201cModel\u2010Free Time\u2010Invariant Evaluation of Transport and Efficacy of Chemicals and Drugs \u201d US Pat Appl. 2024\/0047019 filed July 28 and issued February 8 2022."}],"container-title":["Advanced Sensor Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adsr.202500166","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adsr.202500166","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adsr.202500166","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T05:07:59Z","timestamp":1773205679000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adsr.202500166"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/adsr.202500166"],"URL":"https:\/\/doi.org\/10.1002\/adsr.202500166","archive":["Portico"],"relation":{},"ISSN":["2751-1219","2751-1219"],"issn-type":[{"value":"2751-1219","type":"print"},{"value":"2751-1219","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-10-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00166"}}