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In the case of ocular infections, antibiotic eye drops are commonly prescribed. However, their efficacy is usually compromised by the low viscosity of these formulations and the eye drainage. To overcome these drawbacks, deep eutectic solvent (DES)\u2010based microemulsions with thermo\u2010responsive character, that increase their viscosity upon contact with the eye have been developed. Using betaine\u2010based DES aqueous solutions, it is possible to increase up to 140\u2010fold the water solubility of the antibiotic chloramphenicol, typically used in ocular infections. The DES solutions containing the antibiotic are applied as water phases in water\u2010in\u2010oil\u2010in\u2010water (w\/o\/w) microemulsions, being stable up to 3 months. Furthermore, a sustained\u2010release and a higher permeation of the antibiotic through the cornea than that of commercialized eye drops is achieved, while presenting comparable cytotoxicity profiles (cell viabilities &gt; 88%). Higher antimicrobial activity and faster action of the antibiotic in case of infection with MRSA is observed compared to the commercialized formulations (7 log<jats:sub>10<\/jats:sub> of inactivation in 48\u00a0h vs 72\u00a0h). Overall, these microemulsions comprising DES are a promising strategy to achieve higher antibiotic effectiveness in the treatment of resistant bacterial infections.<\/jats:p>","DOI":"10.1002\/adtp.202200235","type":"journal-article","created":{"date-parts":[[2023,3,4]],"date-time":"2023-03-04T05:14:51Z","timestamp":1677906891000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Thermo\u2010Responsive Microemulsions Containing Deep Eutectic\u2010Based Antibiotic Formulations for Improved Treatment of Resistant Bacterial Ocular Infections"],"prefix":"10.1002","volume":"6","author":[{"given":"S\u00f3nia N.","family":"Pedro","sequence":"first","affiliation":[{"name":"CICECO\u2010Aveiro Institute of Materials Department of Chemistry University of Aveiro  Aveiro 3810\u2010193 Portugal"}]},{"given":"Ana T. P. 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