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Herein, the fabrication of a microneedle (MN) platform is reported using a stereolithography 3D printer, varying the 3D printing angle and aspect ratio (2:1, 3:1, and 4:1). The optimal printing angle is 30\u00b0, resulting in needle tip and base diameters of \u224850 and \u2248330\u00a0\u00b5m and heights of \u2248550\/850\/1180\u00a0\u00b5m. Polyvinyl alcohol (PVA) MNs produced with varying levofloxacin concentrations show variability of \u22484% in tip and 3% base diameters and 15% in height compared to the 3D\u2010printed MNs. Geometry B is used to produce levofloxacin\u2010loaded PVA MNs and tested against <jats:italic>Klebsiella pneumoniae<\/jats:italic> colony biofilms. Levofloxacin is released gradually, as assessed by spectrofluorimetry. The minimum inhibitory concentration of levofloxacin against the <jats:italic>K. pneumoniae<\/jats:italic> clinical isolate is 4\u00a0\u00b5g\u00a0mL<jats:sup>\u22121<\/jats:sup> but this concentration is insufficient to cause any effect on <jats:italic>K. pneumoniae<\/jats:italic> biofilms. Only concentrations \u226532\u00a0\u00b5g\u00a0mL<jats:sup>\u22121<\/jats:sup> are statistically different compared to the unloaded MNs. 3D printing is an attractive solution to produce molds for fabricating biopolymeric MNs for topical drug delivery.<\/jats:p>","DOI":"10.1002\/adtp.202200320","type":"journal-article","created":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T08:43:36Z","timestamp":1680857016000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Levofloxacin\u2010Loaded Microneedles Produced Using 3D\u2010Printed Molds for <i>Klebsiella Pneumoniae<\/i> Biofilm Control"],"prefix":"10.1002","volume":"6","author":[{"given":"K. B.","family":"Vinayakumar","sequence":"first","affiliation":[{"name":"INL\u2014International Iberian Nanotechnology Laboratory  Av. 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