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Subsequent reaction with dipyridyl tetrazine leads to bond cleavage and formation of a pyrrole\u2010linked conjugate. The latter involves ligation of the tetrazine to the azanorbornadiene\u2010tagged protein through inverse electron demand Diels\u2013Alder cycloaddition with subsequent double retro\u2010Diels\u2013Alder reactions to form a stable pyrrole linkage. The sequence of site\u2010selective bioconjugation followed by bioorthogonal bond cleavage was efficiently employed for the labelling of three different proteins. 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