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The influence of the enzyme concentration, the carbodiimide concentration, and the coupling medium on the immobilization reaction was studied. A system with 160 mg of coupled enzyme per gram copolymer, providing 126 mg of active enzyme per gram of copolymer was obtained. The <jats:italic>K<\/jats:italic><jats:sub><jats:italic>M<\/jats:italic><\/jats:sub>, <jats:italic>V<\/jats:italic><jats:sub><jats:italic>max<\/jats:italic><\/jats:sub>, and the optimum temperature profile and optimum pH value for the free and immobilized \u03b1\u2010chymotrypsin were determined.<\/jats:p>","DOI":"10.1002\/app.1990.070410722","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T12:35:25Z","timestamp":1050410125000},"page":"1629-1639","source":"Crossref","is-referenced-by-count":12,"title":["Immobilization of \u03b1\u2010chymotrypsin onto hydrolyzed poly(ethylene)\u2010<i>g<\/i>\u2010co\u2010hydroxyethyl methacrylate"],"prefix":"10.1002","volume":"41","author":[{"given":"M. Alves","family":"Da Silva","sequence":"first","affiliation":[]},{"given":"M. H.","family":"Gil","sequence":"additional","affiliation":[]},{"given":"A. 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