{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:27:11Z","timestamp":1774564031859,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,1,26]],"date-time":"2005-01-26T00:00:00Z","timestamp":1106697600000},"content-version":"vor","delay-in-days":4043,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have used intratracheal instillation of bleomycin in rats to study the microanatomical changes of blood vessels associated with lung fibrosis. Bleomycin is a toxic cytostatic drug employed in classical models of lung fibrosis. Wistar rats were submitted to intratracheal injection of 1.5 units of bleomycin and sacrificed 2.5 months later, a timing when marked fibrosis of the lung is observed. We casted the vascular tree of the rat lungs by perfusion with a methacrylate resin. These caste were studied by scanning electron microscopy. Lung tissue was also studied by light microscopy and thin section electron microscopy. The major vascular modifications observed in the bleomycin\u2010treated rats were: (1) neoformation of an elaborate network of vessels located in the peribronchial domains of the lung, and (2) distortion of the architecture of alveolar capillaries. By light microscopy, it was clear that the newly formed vascular network was located in regions of fibrosis (which in the resin casts were digested away). These neoformed vessels appeared to originate from bronchial arteries. Thin section electron microscopy revealed that endothelial cells of the neoformed vessels were plump, presented large nuclei, and showed numerous pinocytotic vesicles that were also observed in subendothelial pericytes. The alveoli of the bleomycin\u2010treated rats were heterogeneous in size and shape in contrast with the homogeneity of alveoli of control animals. The alveolar capillaries of fibrotic lungs appeared to occupy a larger volume of the alveolar wall than alveolar capillaries of control rats. Our findings indicate that lung fibrosis encompasses marked changes of the vascular system, namely, the neoformation of vessels and the rearrangement of alveolar capillaries. These structural changes suggest that fibrotic transformation of the lung is associated with the local generation of angiogenic stimuli. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.1092380108","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T19:16:49Z","timestamp":1109445409000},"page":"57-67","source":"Crossref","is-referenced-by-count":74,"title":["Neoformation of blood vessels in association with rat lung fibrosis induced by bleomycin"],"prefix":"10.1002","volume":"238","author":[{"given":"M\u00e1rio N. D.","family":"Pe\u00e3o","sequence":"first","affiliation":[]},{"given":"Artur P.","family":"\u00c1guas","sequence":"additional","affiliation":[]},{"given":"Carlos M.","family":"de S\u00e1","sequence":"additional","affiliation":[]},{"given":"Nuno R.","family":"Grande","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,1,26]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"473","article-title":"Contractile cells in normal and fibrotic lung","volume":"60","author":"Adler K. B.","year":"1989","journal-title":"Lab. Invest."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520298209066530"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092330410"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000398298"},{"key":"e_1_2_1_6_1","first-page":"315","article-title":"Interpretation of structural patterns appearing on corrosion casts of small blood and initial lymphatic vessels","volume":"3","author":"Castenholz A.","year":"1989","journal-title":"Scanning Microsc."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00429611"},{"key":"e_1_2_1_8_1","first-page":"767","volume-title":"The Lung: Scientific Foundations Crystal RG","author":"deMello D.","year":"1991"},{"key":"e_1_2_1_9_1","first-page":"16","article-title":"Alveolar\u2010capillary membrane in idiopathic interstitial fibrosis","volume":"98","author":"Gracey D. R.","year":"1968","journal-title":"Am. Rev. Respir. Dis."},{"key":"e_1_2_1_10_1","first-page":"575","article-title":"The characteristic structural features of the blood vessels of the Lewis lung carcinoma","volume":"2","author":"Grunt T. W.","year":"1986","journal-title":"Scanning Microsc."},{"key":"e_1_2_1_11_1","first-page":"557","article-title":"The angioarchitecture of the Lewis lung carcinoma in laboratory mice","volume":"2","author":"Grunt T. W.","year":"1986","journal-title":"Scanning Microsc."},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02034932"},{"key":"e_1_2_1_13_1","first-page":"245","article-title":"Ultrastructural study of lung injury induced by bleomycin sulfate in rats","volume":"11","author":"Hijiya K.","year":"1978","journal-title":"J. Clin. Electron Microsc."},{"key":"e_1_2_1_14_1","first-page":"542","article-title":"New findings in pulmonary arteries of rats with hypoxia\u2010induced pulmonary hipertension","volume":"57","author":"Hislop A.","year":"1976","journal-title":"Br. J. Exp. Pathol."},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0007-0971(78)90061-X"},{"key":"e_1_2_1_16_1","first-page":"889","article-title":"Scanning electron microscopy of vascular corrosion casts: technique and applications","volume":"4","author":"Lametschwandtner A.","year":"1990","journal-title":"Scanning Microsc."},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00705300"},{"key":"e_1_2_1_18_1","first-page":"349","article-title":"Microvasculature of the rat lung as revealed by scanning electron microscopy","volume":"3","author":"Ohtani O.","year":"1980","journal-title":"Scan. Electron. Microsc."},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/01902149209031712"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00203719"},{"key":"e_1_2_1_21_1","first-page":"377","article-title":"Anatomy of Clara cell secretion: Surface changes captured by scanning electron microscopy","volume":"183","author":"Pe\u00e3o M. N. D.","year":"1993","journal-title":"J. Anat."},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","unstructured":"Pe\u00e3o M. N. D. A. P.\u00c1guas C. M.de S\u00e1 andN. R.Grande1993dMorphological evidence for migration of particle\u2010laden macrophages through the interalveolar pores of Kohn in the murine lung.Acta Anatomica(in press).","DOI":"10.1159\/000147509"},{"key":"e_1_2_1_23_1","first-page":"42","article-title":"Scanning electron microscopy of the deep lymphatic network of the murin lung as viewed in corrosion casts","volume":"26","author":"Pe\u00e3o M. N. D.","year":"1993","journal-title":"Lympholog."},{"key":"e_1_2_1_24_1","first-page":"281","article-title":"Structural artifacts and advantages of cytocentrifugation of cells as viewed by scanning electron microscopy","volume":"6","author":"Pe\u00e3o M. N. D.","year":"1992","journal-title":"Scanning Microsc."},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198305000-00012"},{"key":"e_1_2_1_26_1","doi-asserted-by":"crossref","first-page":"774","DOI":"10.4269\/ajtmh.1982.31.779","article-title":"Pulmonary changes in schistosomal cor pulmonale","volume":"31","author":"Sadigursky M.","year":"1982","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_2_1_27_1","first-page":"1733","article-title":"Microvascular corrosion casting of the lung. A state\u2010of\u2010the\u2010art review","volume":"1","author":"Schraufnagel D. E.","year":"1987","journal-title":"Scanning Microsc."},{"key":"e_1_2_1_28_1","first-page":"575","article-title":"Microvascular casting of the lung: Bronchial versus pulmonary filling","volume":"3","author":"Schraufnagel D. E.","year":"1989","journal-title":"Scanning Microsc."},{"key":"e_1_2_1_29_1","first-page":"1083","article-title":"Monocrotaline\u2010induced angiogenesis: differences in the bronchial and pulmonary vasculature","volume":"137","author":"Schraufnagel D. E.","year":"1990","journal-title":"Am. J Pathol."},{"key":"e_1_2_1_30_1","first-page":"126","article-title":"Capillary structure in pulmonary emphysema","volume":"130","author":"Schraufnagel D. E.","year":"1988","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_31_1","first-page":"97","article-title":"Capillary remodeling in bleomycin\u2010induced pulmonary fibrosis","volume":"125","author":"Schraufnagel D. E.","year":"1986","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_32_1","doi-asserted-by":"crossref","first-page":"2006","DOI":"10.1128\/iai.55.9.2006-2016.1987","article-title":"In vivo killing and degradation of Mycobacterium aurum within mouse peritoneal macrophages","volume":"55","author":"Silva M. T.","year":"1987","journal-title":"Infect. Immun."},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(76)90068-2"},{"key":"e_1_2_1_34_1","first-page":"289","article-title":"Chronic interstitial pulmonary fibrosis produced in hamsters by endotracheal bleomycin: pathology and stereology","volume":"117","author":"Snider G. L.","year":"1978","journal-title":"Am. Rev. Respir. Dis."},{"key":"e_1_2_1_35_1","first-page":"117","article-title":"Bleomycin\u2010induced pulmonary fibrosis in the rat","volume":"95","author":"Thrall R. S.","year":"1979","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.18.3.225"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.1092380108","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.1092380108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T02:18:00Z","timestamp":1698113880000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ar.1092380108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,1]]}},"alternative-id":["10.1002\/ar.1092380108"],"URL":"https:\/\/doi.org\/10.1002\/ar.1092380108","archive":["Portico"],"relation":{},"ISSN":["0003-276X","1097-0185"],"issn-type":[{"value":"0003-276X","type":"print"},{"value":"1097-0185","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,1]]}}}