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These studies are essential for the evaluation of the potential therapeutic effect of drugs commonly used in the treatment of Alzheimer's disease. Donepezil, galantamine, and rivastigmine were tested, in addition to compounds based on the xanthone scaffold. Four of these xanthone derivatives were identified as having EC<jats:sub>50<\/jats:sub> values between 676 and 4466\u2009\u00b5mol\/l, showing a potential inhibitory effect higher than the clinical agent rivastigmine. The developed automatic system added advantages of reduction of reagents and sample consumption (around 55\u2009\u00b5l per analysis), lower cost per analysis, and the generation of less waste (around 1.2\u2009ml per analysis). The \u00b5SIA\u2013LOV system is also a robust, rapid, reliable, and simple system to use. Docking studies suggested a possible mode of interaction with the target acetylcholinesterase protein.<\/jats:p>","DOI":"10.1002\/ardp.202100150","type":"journal-article","created":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T13:16:25Z","timestamp":1623244585000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microsequential injection analysis\/lab\u2010on\u2010valve system for the automatic evaluation of acetylcholinesterase inhibitors"],"prefix":"10.1002","volume":"354","author":[{"given":"Thierno Moussa","family":"Ndongo","sequence":"first","affiliation":[{"name":"LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy Porto University Porto Portugal"},{"name":"Facult\u00e9 des sciences et techniques Le Mans Universit\u00e9 Le Mans France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1383-4405","authenticated-orcid":false,"given":"Marieta L. 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