{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T18:44:41Z","timestamp":1772045081239,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,4,26]],"date-time":"2025-04-26T00:00:00Z","timestamp":1745625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biofuels Bioprod Bioref"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microbial lipids produced by yeasts from lignocellulosic biomass are a promising feedstock for the biodiesel industry, providing a renewable energy source as an alternative to traditional fossil fuels. This study investigated the potential of <jats:italic>Yarrowia lipolytica<\/jats:italic> W29 to produce lipid\u2010rich biomass from undetoxified sugar\u2010concentrated eucalyptus bark hydrolysate (EBH). The lipid concentrations achieved in batch cultures (13.4\u2009g L<jats:sup>\u22121<\/jats:sup>) were the highest for wild\u2010type <jats:italic>Y. lipolytica<\/jats:italic> strains in lignocellulosic hydrolysates. Different two\u2010stage cultivation modes (repeated batch, continuous\u2010feeding fed\u2010batch, and pulse fed\u2010batch) were studied to enhance biomass and lipid production. The cell and lipid mass was higher in pulse fed\u2010batch and continuous\u2010feeding fed\u2010batch cultures than batch cultures. Production of citric acid, a side product of industrial interest, was improved in the continuous\u2010feeding fed\u2010batch culture. Microbial lipids produced by <jats:italic>Y. lipolytica<\/jats:italic> W29 were highly unsaturated and mainly composed of oleic acid (50% to 53%). The estimated properties of the biodiesel that would be obtained from these intracellular lipids would meet the international biodiesel standards EN 14214 and ASTM D6751. This study demonstrates the feasibility of using EBH for <jats:italic>Y. lipolytica<\/jats:italic> lipid production and promotes the sustainable production of high\u2010quality biodiesel from lignocellulosic feedstocks.<\/jats:p>","DOI":"10.1002\/bbb.2786","type":"journal-article","created":{"date-parts":[[2025,4,26]],"date-time":"2025-04-26T03:35:14Z","timestamp":1745638514000},"page":"1259-1270","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Alternative feedstocks for high\u2010quality biodiesel: Lipid production from eucalyptus bark hydrolysate by <i>Yarrowia lipolytica<\/i> W29 using different cultivation modes"],"prefix":"10.1002","volume":"19","author":[{"given":"Bruna","family":"Dias","sequence":"first","affiliation":[{"name":"CEB \u2013 Centre of Biological Engineering University of Minho  Braga Portugal"}]},{"given":"Marlene","family":"Lopes","sequence":"additional","affiliation":[{"name":"CEB \u2013 Centre of Biological Engineering University of Minho  Braga Portugal"},{"name":"LABBELS \u2013 Associate Laboratory  Braga\/Guimar\u00e3es Portugal"}]},{"given":"Susana","family":"Marques","sequence":"additional","affiliation":[{"name":"Unit of Bioenergy and Biorefineries, LNEG  Lisbon Portugal"}]},{"given":"Francisco","family":"G\u00edrio","sequence":"additional","affiliation":[{"name":"Unit of Bioenergy and Biorefineries, LNEG  Lisbon Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6747-8389","authenticated-orcid":false,"given":"Isabel","family":"Belo","sequence":"additional","affiliation":[{"name":"CEB \u2013 Centre of Biological Engineering University of Minho  Braga Portugal"},{"name":"LABBELS \u2013 Associate Laboratory  Braga\/Guimar\u00e3es Portugal"}]}],"member":"311","published-online":{"date-parts":[[2025,4,26]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"1","article-title":"Microbial biodiesel production from lignocellulosic biomass: new insights and future challenges biomass","volume":"52","author":"Uthandi S","year":"2021","journal-title":"Crit Rev Environ Sci Technol"},{"issue":"100167","key":"e_1_2_6_3_1","first-page":"1","article-title":"Biodiesel production from lignocellulosic biomass using Yarrowia lipolytica","volume":"13","author":"Vasaki M","year":"2022","journal-title":"Energy Convers Manag"},{"issue":"113043","key":"e_1_2_6_4_1","first-page":"1","article-title":"Factors affecting the optimisation and scale\u2010up of lipid accumulation in oleaginous yeasts for sustainable biofuels production","volume":"171","author":"Robles\u2010Iglesias R","year":"2023","journal-title":"Renew Sust Energ Rev"},{"key":"e_1_2_6_5_1","volume-title":"Renewables 2020 \u2013 Analysis and Forecast to 2025","author":"IEA","year":"2020"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apenergy.2011.10.031"},{"key":"e_1_2_6_7_1","unstructured":"McCormickRandMoriartyK Biodiesel Handling and Use Guide [Internet](2023). 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