{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T13:53:17Z","timestamp":1765806797028,"version":"build-2065373602"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,5,5]],"date-time":"2009-05-05T00:00:00Z","timestamp":1241481600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The main objective of this work was studying and testing the nature and influence of reaction media (organic solvent <jats:italic>vs.<\/jats:italic> miniemulsion system) on the synthesis of alkyl esters catalyzed by <jats:italic>Fusarium solani pisi<\/jats:italic> cutinase. Ester synthesis and cutinase selectivity for different chain length of acids and alcohols (ethyl and hexyl) were evaluated. In <jats:italic>iso<\/jats:italic>\u2010octane, after 1 h of reaction, cutinase exhibits rates of esterification between 0.24 \u03bcmol x mg<jats:sup>\u2013<\/jats:sup>1 x min<jats:sup>\u20131<\/jats:sup> for ethyl oleate and 1.15 \u03bcmol x mg<jats:sup>\u2013<\/jats:sup>1 x min<jats:sup>\u20131<\/jats:sup> for ethyl butyrate, while in a miniemulsion system the rates were from 0.05 for ethyl heptanoate to 0.76 \u03bcmol x mg<jats:sup>\u20131<\/jats:sup> x min<jats:sup>\u20131<\/jats:sup> for ethyl decanoate. The reaction rate for the synthesis of hexyl esters in a miniemulsion system was from 0.19 for hexyl heptanoate to 1.07 \u03bcmol x mg<jats:sup>\u2013<\/jats:sup>1 x min<jats:sup>\u20131<\/jats:sup> for hexyl decanoate. High conversion yields of 95% at equilibrium after 8 h of reaction in <jats:italic>iso<\/jats:italic>\u2010octane for pentanoic acid (C<jats:sub>5<\/jats:sub>) with ethanol at equimolar concentration (0.1 M) was achieved. Additionally, this work showed that a significant and unexpected shift in cutinase selectivity occurred towards longer chain length carboxylic acids (C<jats:sub>8<\/jats:sub>\u2013C<jats:sub>10<\/jats:sub>) in miniemulsion system as compared to organic solvent (<jats:italic>iso<\/jats:italic>\u2010octane) and previous studies in reverse micellar systems. The possibility of working with higher concentration of substrates, without inhibitory effect on the enzyme, was another advantage of the miniemulsion system.<\/jats:p>","DOI":"10.1002\/biot.200800294","type":"journal-article","created":{"date-parts":[[2009,5,5]],"date-time":"2009-05-05T08:13:37Z","timestamp":1241511217000},"page":"674-683","source":"Crossref","is-referenced-by-count":23,"title":["Synthesis of alkyl esters by cutinase in miniemulsion and organic solvent media"],"prefix":"10.1002","volume":"4","author":[{"given":"Dragana P. C.","family":"de Barros","sequence":"first","affiliation":[]},{"given":"Lu\u00eds P.","family":"Fonseca","sequence":"additional","affiliation":[]},{"given":"Joaquim M. 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