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In this work, quartz crystal microbalance (QCM) devices were used to follow the adhesion of NIH 3T3 fibroblasts to QCM surfaces modified with fibronectin (FN) and poly\u2010<jats:sc>D<\/jats:sc>\u2010lysine (PDL). The variations in sensor resonant frequency (\u0394<jats:italic>f<\/jats:italic>) and motional resistance (\u0394R), monitored as the sensor signal, revealed that cell adhesion was favored in the PDL\u2010coated QCMs. Fluorescence microscopy images of seeded cells showed more highly spread cells on the PDL substrate, which is consistent with the results of the QCM signals. The sensor signal was shown to be sensitive to extracellular matrix (ECM)\u2010binding motifs. Ethylenediaminetetraacetic acid (EDTA) and soluble Gly\u2010Arg\u2010Gly\u2010Asp\u2010Ser (GRGDS) peptides were used to interfere with cell\u2010ECM binding motifs onto FN\u2010coated QCMs. The acquired acoustic signals successfully showed that in the presence of 30 mM EDTA or 1 mM GRGDS, cell adhesion is almost completely abolished due to the inhibition\/blocking of integrin function by these compounds. The results presented here demonstrate the potential of the QCM sensor to study cell adhesion, to monitor the biocompatibility of polymers and materials, and to assess the effect of adhesion modulators. QCM sensors have great potential in tissue engineering applications, as QCM sensors are able to analyze the biocompatibility of surfaces and it has the added advantage of being able to evaluate, in situ and in real time, the effect of specific drugs\/treatments on cells.<\/jats:p>","DOI":"10.1002\/biot.201200320","type":"journal-article","created":{"date-parts":[[2013,2,28]],"date-time":"2013-02-28T21:02:13Z","timestamp":1362085333000},"page":"690-698","source":"Crossref","is-referenced-by-count":12,"title":["Acoustic detection of cell adhesion to a coated quartz crystal microbalance \u2013 implications for studying the biocompatibility of polymers"],"prefix":"10.1002","volume":"8","author":[{"given":"Ana\u2010Carina","family":"Da\u2010Silva","sequence":"first","affiliation":[]},{"given":"Sandra S.","family":"Soares","sequence":"additional","affiliation":[]},{"given":"Guilherme N. 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