{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T01:45:30Z","timestamp":1776735930097,"version":"3.51.2"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,4,11]],"date-time":"2013-04-11T00:00:00Z","timestamp":1365638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Monoclonal antibodies (mAbs) are important therapeutic proteins. One of the challenges facing large\u2010scale production of monoclonal antibodies is the capacity bottleneck in downstream processing, which can be circumvented by using magnetic stimuli\u2010responsive polymer nanoparticles. In this work, stimuli\u2010responsive magnetic particles composed of a magnetic poly(methyl methacrylate) core with a poly(N\u2010isopropylacrylamide\u2010co\u2010acrylic acid) (P(NIPAM\u2010co\u2010AA)) shell cross\u2010linked with N, N'\u2010methylenebisacrylamide were prepared by miniemulsion polymerization. The particles were shown to have an average hydrodynamic diameter of 317 nm at 18\u00b0C, which decreased to 277 nm at 41\u00b0C due to the collapse of the thermo\u2010responsive shell. The particles were superparamagnetic in behavior and exhibited a saturation magnetization of 12.6 emu\/g. Subsequently, we evaluated the potential of these negatively charged stimuli\u2010responsive magnetic particles in the purification of a monoclonal antibody from a diafiltered CHO cell culture supernatant by cation exchange. The adsorption of antibodies onto P(NIPAM\u2010co\u2010AA)\u2010coated nanoparticles was highly selective and allowed for the recovery of approximately 94% of the mAb. Different elution strategies were employed providing highly pure mAb fractions with host cell protein (HCP) removal greater than 98%. By exploring the stimuli\u2010responsive properties of the particles, shorter magnetic separation times were possible without significant differences in product yield and purity.<\/jats:p>","DOI":"10.1002\/biot.201200329","type":"journal-article","created":{"date-parts":[[2013,2,19]],"date-time":"2013-02-19T12:54:19Z","timestamp":1361278459000},"page":"709-717","source":"Crossref","is-referenced-by-count":29,"title":["Stimuli\u2010Responsive magnetic nanoparticles for monoclonal antibody purification"],"prefix":"10.1002","volume":"8","author":[{"given":"Lu\u00eds","family":"Borlido","sequence":"first","affiliation":[]},{"given":"Leila","family":"Moura","sequence":"additional","affiliation":[]},{"given":"Ana M.","family":"Azevedo","sequence":"additional","affiliation":[]},{"given":"Ana C. A.","family":"Roque","sequence":"additional","affiliation":[]},{"given":"Maria R.","family":"Aires\u2010Barros","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 Paulo S.","family":"Farinha","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2013,4,11]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1093\/clinchem\/46.9.1478","article-title":"Bioconjugates of intelligent polymers and recognition proteins for use in diagnostics and affinity separations.","volume":"46","author":"Hoffman A. S.","year":"2000","journal-title":"Clin. Chem."},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmat2614"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2009.10.004"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-003-1330-7"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2011.07.030"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2006.06.021"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2007.08.012"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-8853(00)01244-0"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/bp070185e"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cphc.201000015"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp804747b"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1351\/PAC-CON-08-11-11"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00166089"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3900(200002)151:1<617::AID-MASY617>3.0.CO;2-T"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac201814n"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/la2045477"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2004.10.012"},{"key":"e_1_2_7_19_2","doi-asserted-by":"crossref","unstructured":"Gu H. Xu K. Xu C. Xu B. Biofunctional magnetic nanoparticles for protein separation and pathogen detection.Chem. Commun.2006 941\u2013949.","DOI":"10.1039\/b514130c"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200602866"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.200902274"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201000260"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp208788k"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0cc00637h"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200802469"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0jm00565g"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.200801844"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp807081b"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/ie800969q"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.200801689"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200803159"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200905133"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja076086e"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/smll.200901596"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201003602"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200802260"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0711440"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.08.084"},{"key":"e_1_2_7_39_2","unstructured":"Borlido L. Azevedo A. M. Sousa A. G. Oliveira P. H. et al. Fishing human monoclonal antibodies from a cho cell supernatant with boronic acid magnetic particles.J. Chromatogr. B."},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/pola.21391"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr068445e"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/1099-0518(20001215)38:24<4441::AID-POLA130>3.0.CO;2-U"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/pola.21525"},{"key":"e_1_2_7_45_2","doi-asserted-by":"publisher","DOI":"10.1021\/la702281k"},{"key":"e_1_2_7_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/macp.200290052"},{"key":"e_1_2_7_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2009.08.052"},{"key":"e_1_2_7_48_2","doi-asserted-by":"publisher","DOI":"10.1006\/jcis.1993.1075"},{"key":"e_1_2_7_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/0001-8686(94)00222-X"},{"key":"e_1_2_7_50_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.matsci.36.011205.123513"},{"key":"e_1_2_7_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.07.077"}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiot.201200329","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.201200329","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:13:33Z","timestamp":1759940013000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.201200329"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,11]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1002\/biot.201200329"],"URL":"https:\/\/doi.org\/10.1002\/biot.201200329","archive":["Portico"],"relation":{},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"value":"1860-6768","type":"print"},{"value":"1860-7314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,11]]}}}