{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T21:56:56Z","timestamp":1765231016184,"version":"build-2065373602"},"reference-count":73,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T00:00:00Z","timestamp":1409270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Silk fibroin demonstrates great biocompatibility and is suitable for many biomedical applications, including tissue engineering and regenerative medicine. Current research focuses on manipulating the physico\u2010chemical properties of fibroin, and examining the effect of this manipulation on firobin's biocompatibility. Regenerated silk fibroin was modified by in vitro enzymatic phosphorylation and cast into films. Films were produced by blending, at several ratios, the phosphorylated and un\u2010phosphorylated fibroin solutions. Fourier transform infra\u2010red spectroscopy was used to determine the specific P\u2013OH vibration peak, confirming the phosphorylation of the regenerated silk fibroin solution. Differential scanning calorimetry showed that phosphorylation altered the intra\u2010 and inter\u2010molecular interactions. Further experiments demonstrated that phosphorylation can be used to tailor the hydrophylicity\/hydrophobicity ratio as well as the crystalinity of silk fibroin films. Release profiling of a model drug was highly dependent on silk modification level. Cytotoxicity assays showed that exposure to lixiviates of phosphorylated films only slightly affected cellular metabolism and proliferation, although direct contact resulted in a strong direct correlation between phosphorylation level and cell proliferation. This new method for tuning silk biomaterials to obtain specific structural and biochemical features can be adapted for a wide range of applications. Phosphorylation of silk fibroins may be applied to improve the cytocompatibility of any silk\u2010based device that is considered to be in contact with live animals or human tissues.<\/jats:p>","DOI":"10.1002\/biot.201400302","type":"journal-article","created":{"date-parts":[[2014,8,3]],"date-time":"2014-08-03T18:42:19Z","timestamp":1407091339000},"page":"1267-1278","source":"Crossref","is-referenced-by-count":9,"title":["Phosphorylation of silk fibroins improves the cytocompatibility of silk fibroin derived materials: A platform for the production of tuneable material"],"prefix":"10.1002","volume":"9","author":[{"given":"Vadim","family":"Volkov","sequence":"first","affiliation":[]},{"given":"Andreia","family":"Vasconcelos","sequence":"additional","affiliation":[]},{"given":"Marisa P.","family":"S\u00e1rria","sequence":"additional","affiliation":[]},{"given":"Andreia C.","family":"Gomes","sequence":"additional","affiliation":[]},{"given":"Artur","family":"Cavaco\u2010Paulo","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2014,8,29]]},"reference":[{"key":"e_1_2_7_2_2","first-page":"717","article-title":"Silk polymers: Material science and biotechnology.","volume":"6","author":"Kaplan D.","year":"1995","journal-title":"Polym. Adv. Technol."},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(99)00088-6"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.1078"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/bm300089a"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2007.05.013"},{"key":"e_1_2_7_7_2","doi-asserted-by":"crossref","unstructured":"Lawrence B. D. Processing of Bombyx mori silk for biomedical applications in: Kundu S. (Ed.) Silk Biomaterials for Tissue Engineering and Regenerative Medicine Elsevier 2014 pp. 84\u201393.","DOI":"10.1533\/9780857097064.1.78"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2010.03.009"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2011.05.017"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2012.12.038"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.201000173"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.30186"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(02)00326-5"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2006.03.003"},{"key":"e_1_2_7_15_2","first-page":"85","article-title":"Molecular biology of spider silk.","volume":"74","author":"Winkler S.","year":"2000","journal-title":"J. Biotechnol."},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja063019w"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2434996"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi005119z"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr000230w"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.3109\/10409238409102298"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.2492\/inflammregen.31.137"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-8-59"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2007.08.037"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2004.01.011"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.07.044"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M700574-MCP200"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki471"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-163"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)00503-1"},{"key":"e_1_2_7_30_2","doi-asserted-by":"crossref","unstructured":"Samuel D. Silver B. L. Elimination reactions and hydrolysis of serine phosphate.J. Chem. Soc.1963 289\u2013296.","DOI":"10.1039\/jr9630000289"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(91)90194-X"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2007.06.048"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2001.2206"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7270.2007.00320.x"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2011.11.050"},{"key":"e_1_2_7_36_2","first-page":"23","article-title":"A simple equation for description of solute release I. Fickian and non\u2010fickian release from non\u2010swellable devices in the form of slabs, spheres, cylinders or discs.","volume":"5","author":"Philip L.","year":"1986","journal-title":"J. Controlled Release"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600521210"},{"key":"e_1_2_7_38_2","first-page":"129","article-title":"Cytotoxicity of current adhesive systems: in vitro testing on cell culture of L929 and balb\/c 3T3 fibroblasts.","volume":"24","author":"Poskus L. T.","year":"2009","journal-title":"Rev. odonto. cienc."},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-894X(01)81162-8"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/82.13.1107"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2004.12.056"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/polb.1994.090320519"},{"key":"e_1_2_7_43_2","first-page":"1273","article-title":"Investigation of the structure of silk film regenerated with lithium thiocyanate solution.","volume":"21","author":"N. V. Bhat S. M. A.","year":"1983","journal-title":"J. Polym. Sci., Part A: Polym. Chem."},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1366\/000370206779321355"},{"key":"e_1_2_7_45_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.10241"},{"key":"e_1_2_7_46_2","doi-asserted-by":"publisher","DOI":"10.1021\/bm050010y"},{"key":"e_1_2_7_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813012106"},{"key":"e_1_2_7_48_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3935(200207)203:10\/11<1658::AID-MACP1658>3.0.CO;2-3"},{"key":"e_1_2_7_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf101010w"},{"key":"e_1_2_7_50_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867054546564"},{"key":"e_1_2_7_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/pol.1977.180150915"},{"key":"e_1_2_7_52_2","doi-asserted-by":"publisher","DOI":"10.1002\/app.1975.070190410"},{"key":"e_1_2_7_53_2","doi-asserted-by":"publisher","DOI":"10.1039\/c1ay05493g"},{"key":"e_1_2_7_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-0987(01)00095-1"},{"key":"e_1_2_7_55_2","first-page":"148","article-title":"In\u2010vitro Release of Metformin Hydrochloride from Films of Chitosan\u2010Methylcellulose Blends.","volume":"21","author":"Kamlesh K. C. S.","year":"2009","journal-title":"Asian J. Chem."},{"key":"e_1_2_7_56_2","doi-asserted-by":"publisher","DOI":"10.1214\/aoms\/1177730491"},{"key":"e_1_2_7_57_2","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.B0900163"},{"key":"e_1_2_7_58_2","doi-asserted-by":"publisher","DOI":"10.1163\/092050610X530964"},{"key":"e_1_2_7_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00356-9"},{"key":"e_1_2_7_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/0267-6605(91)90004-Y"},{"key":"e_1_2_7_61_2","doi-asserted-by":"publisher","DOI":"10.1021\/jz300103p"},{"key":"e_1_2_7_62_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja808999m"},{"key":"e_1_2_7_63_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja1111964"},{"key":"e_1_2_7_64_2","first-page":"123","article-title":"Theoretical pKa prediction of O\u2010phosphoserine in aqueous solution.","volume":"514","author":"Smiechowski M.","year":"2011","journal-title":"Chem. Phys. Lett."},{"key":"e_1_2_7_65_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00339-005-3426-7"},{"key":"e_1_2_7_66_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1294"},{"key":"e_1_2_7_67_2","doi-asserted-by":"publisher","DOI":"10.1002\/hlca.19950780304"},{"key":"e_1_2_7_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2005.12.009"},{"key":"e_1_2_7_69_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0jm01668c"},{"key":"e_1_2_7_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2006.06.002"},{"key":"e_1_2_7_71_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32057"},{"key":"e_1_2_7_72_2","doi-asserted-by":"publisher","DOI":"10.1039\/c1cs15252a"},{"key":"e_1_2_7_73_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)00001-0"},{"key":"e_1_2_7_74_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4636(20011205)57:3<336::AID-JBM1176>3.0.CO;2-E"}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiot.201400302","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.201400302","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T13:02:43Z","timestamp":1759928563000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.201400302"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,29]]},"references-count":73,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1002\/biot.201400302"],"URL":"https:\/\/doi.org\/10.1002\/biot.201400302","archive":["Portico"],"relation":{},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"type":"print","value":"1860-6768"},{"type":"electronic","value":"1860-7314"}],"subject":[],"published":{"date-parts":[[2014,8,29]]}}}