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A successful example was the eradication of smallpox in 1979 after two centuries of vaccination campaigns. Since the first variolation administrations until today, the knowledge on immunology has increased substantially. This knowledge combined with the introduction of cell culture and DNA recombinant technologies revolutionized vaccine design. This review will focus on vaccines against human viral pathogens, recent developments on vaccine design and cell substrates used for their manufacture. While the production of attenuated and inactivated vaccines requires the use of the respective permissible cell substrates, the production of recombinant antigens, virus\u2010like particles, vectored vaccines and chimeric vaccines requires the use \u2013 and often the development \u2013 of specific cell lines. Indeed, the development of novel modern viral vaccine designs combined with, the stringent safety requirements for manufacture, and the better understanding on animal cell metabolism and physiology are increasing the awareness on the importance of cell line development and engineering areas. A new era of modern vaccinology is arriving, offering an extensive toolbox to materialize novel and creative ideas in vaccine design and its manufacture.<\/jats:p>","DOI":"10.1002\/biot.201400387","type":"journal-article","created":{"date-parts":[[2015,7,26]],"date-time":"2015-07-26T11:17:51Z","timestamp":1437909471000},"page":"1329-1344","source":"Crossref","is-referenced-by-count":63,"title":["Viral vaccines and their manufacturing cell substrates: New trends and designs in modern vaccinology"],"prefix":"10.1002","volume":"10","author":[{"given":"Ana F.","family":"Rodrigues","sequence":"first","affiliation":[]},{"given":"Hugo R.","family":"Soares","sequence":"additional","affiliation":[]},{"given":"Miguel R.","family":"Guerreiro","sequence":"additional","affiliation":[]},{"given":"Paula M.","family":"Alves","sequence":"additional","affiliation":[]},{"given":"Ana S.","family":"Coroadinha","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2015,7,24]]},"reference":[{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1204186"},{"key":"e_1_2_9_3_2","unstructured":"World Health Organization Global Alliance for Vaccines and Immunization(GAVI) Fact sheet169 revised March 2001 http:\/\/www.who.int\/mediacentre\/factsheets\/fs169\/en."},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00377-3"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2002.131098"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/08998280.2005.11928028"},{"key":"e_1_2_9_7_2","unstructured":"Bonanni P. 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