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However, safety concerns have delayed the clinical translation of this technology. One cause for this is the capacity that hPSCs have to generate tumors after transplantation. Because of the challenges associated with achieving complete differentiation into clinically relevant cell types, the development of safe and efficient strategies for purifying committed cells is essential for advancing hPSC\u2010based therapies. Several purification strategies have now succeeded in generating non\u2010tumorigenic and homogeneous cell\u2010populations. These techniques typically enrich for cells by either depleting early committed populations from teratoma\u2010initiating hPSCs or by positively selecting cells after differentiation. Here we review the working principles behind separation methods that have facilitated the safe and controlled application of hPSC\u2010derived cells in laboratory settings and pre\u2010clinical research. 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