{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T22:50:44Z","timestamp":1774392644482,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,2,18]],"date-time":"2015-02-18T00:00:00Z","timestamp":1424217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"FCT","award":["PhD grant SFRH \/ BD \/ 51187 \/ 2010 and SYNBIOBACTHER project (PTDC\/EBB-BIO\/102863\/2008"],"award-info":[{"award-number":["PhD grant SFRH \/ BD \/ 51187 \/ 2010 and SYNBIOBACTHER project (PTDC\/EBB-BIO\/102863\/2008"]}]},{"DOI":"10.13039\/501100008530","name":"FEDER","doi-asserted-by":"crossref","award":["FCOMP-01-0124-FEDER-027462;PEst-OE\/EQB\/LA0023\/2013, NORTE-07-0124-FEDER-000028 and NORTE-07-0124-FED"],"award-info":[{"award-number":["FCOMP-01-0124-FEDER-027462;PEst-OE\/EQB\/LA0023\/2013, NORTE-07-0124-FEDER-000028 and NORTE-07-0124-FED"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Curcuminoids are phenylpropanoids with high pharmaceutical potential. Herein, we report an engineered artificial pathway in <jats:italic>Escherichia coli<\/jats:italic> to produce natural curcuminoids through caffeic acid. <jats:italic>Arabidopsis thaliana<\/jats:italic> 4\u2010coumaroyl\u2010CoA ligase and <jats:italic>Curcuma longa<\/jats:italic> diketide\u2010CoA synthase (DCS) and curcumin synthase (CURS1) were used to produce curcuminoids and 70 mg\/L of curcumin was obtained from ferulic acid. Bisdemethoxycurcumin and demethoxycurcumin were also produced, but in lower concentrations, by feeding <jats:italic>p<\/jats:italic>\u2010coumaric acid or a mixture of <jats:italic>p<\/jats:italic>\u2010coumaric acid and ferulic acid, respectively. Additionally, curcuminoids were produced from tyrosine through the caffeic acid pathway. To produce caffeic acid, tyrosine ammonia lyase from <jats:italic>Rhodotorula glutinis<\/jats:italic> and 4\u2010coumarate 3\u2010hydroxylase from<jats:italic> Saccharothrix espanaensis<\/jats:italic> were used. Caffeoyl\u2010CoA 3\u2010<jats:italic>O<\/jats:italic>\u2010methyltransferase from <jats:italic>Medicago sativa<\/jats:italic> was used to convert caffeoyl\u2010CoA to feruloyl\u2010CoA. Using caffeic acid, <jats:italic>p<\/jats:italic>\u2010coumaric acid or tyrosine as a substrate, 3.9, 0.3, and 0.2 mg\/L of curcumin were produced, respectively. This is the first time DCS and CURS1 were used in vivo to produce curcuminoids and that curcumin was produced by feeding tyrosine. We have shown that curcumin can be produced using a pathway involvoing caffeic acid. This alternative pathway represents a step forward in the heterologous production of curcumin using <jats:italic>E. coli<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/biot.201400637","type":"journal-article","created":{"date-parts":[[2015,1,8]],"date-time":"2015-01-08T07:49:13Z","timestamp":1420703353000},"page":"599-609","source":"Crossref","is-referenced-by-count":58,"title":["Production of curcuminoids from tyrosine by a metabolically engineered <i>Escherichia coli<\/i> using caffeic acid as an intermediate"],"prefix":"10.1002","volume":"10","author":[{"given":"Joana L.","family":"Rodrigues","sequence":"first","affiliation":[]},{"given":"Rafael G.","family":"Ara\u00fajo","sequence":"additional","affiliation":[]},{"given":"Kristala L. 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