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In this work, a series of alternative and biocompatible ABS composed of cholinium\u2010based ILs and polypropylene glycol were investigated. The respective ternary phase diagrams, tie\u2010lines, tie\u2010line lengths and critical points were determined at 25\u00b0C. The extraction performance of these systems for commercial bovine serum albumin (BSA) was then evaluated. The stability of BSA at the IL\u2010rich phase was ascertained by size exclusion high\u2010performance liquid chromatography and Fourier transform infrared spectroscopy. Appropriate ILs lead to the complete extraction of BSA for the IL\u2010rich phase, in a single step, while maintaining the protein's native conformation. Furthermore, to evaluate the performance of these systems when applied to real matrices, the extraction of BSA from bovine serum was additionally carried out, revealing that the complete extraction of BSA was maintained and achieved in a single step. The remarkable extraction efficiencies obtained are far superior to those observed with typical polymer\u2010based ABS. Therefore, the proposed ABS may be envisaged as a more effective and biocompatible approach for the separation and purification of other value\u2010added proteins.<\/jats:p>","DOI":"10.1002\/biot.201500003","type":"journal-article","created":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T09:31:11Z","timestamp":1428658271000},"page":"1457-1466","source":"Crossref","is-referenced-by-count":103,"title":["Enhanced extraction of proteins using cholinium\u2010based ionic liquids as phase\u2010forming components of aqueous biphasic systems"],"prefix":"10.1002","volume":"10","author":[{"given":"Maria V.","family":"Quental","sequence":"first","affiliation":[]},{"given":"Magda","family":"Caban","sequence":"additional","affiliation":[]},{"given":"Matheus M.","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Piotr","family":"Stepnowski","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o A. 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