{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:14:53Z","timestamp":1762431293003,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,7,5]],"date-time":"2017-07-05T00:00:00Z","timestamp":1499212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004963","name":"Seventh Framework Programme","doi-asserted-by":"publisher","award":["EDUFLUVAC project, FP7\u2010HEALTH\u20102013\u2010INNOVATION"],"award-info":[{"award-number":["EDUFLUVAC project, FP7\u2010HEALTH\u20102013\u2010INNOVATION"]}],"id":[{"id":"10.13039\/501100004963","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/52302\/2013"],"award-info":[{"award-number":["SFRH\/BD\/52302\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Virus\u2010like particles (VLPs) are becoming established as vaccines, in particular for influenza pandemics, increasing the interest in the development of VLPs manufacturing bioprocess. However, for complex VLPs, the analytical tools used for quantification are not yet able to keep up with the bioprocess progress. Currently, quantification for Influenza relies on traditional methods: hemagglutination assay or Single Radial Immunodiffusion. These analytical technologies are time\u2010consuming, cumbersome, and not supportive of efficient downstream process development and monitoring. Hereby we report a label\u2010free tool that uses Biolayer interferometry (BLI) technology applied on an Octet platform to quantify Influenza VLPs at all stages of bioprocess. Human (\u03b12,6\u2010linked sialic acid) and avian (\u03b12,3\u2010linked sialic acid) biotinylated receptors associated with streptavidin biosensors were used, to quantify hemagglutinin content in several mono\u2010 and multivalent Influenza VLPs. The applied method was able to quantify hemagglutinin from crude samples up to final bioprocessing VLP product. BLI technology confirmed its value as a high throughput analytical tool with high sensitivity and improved detection limits compared to traditional methods. This simple and fast method allowed for real\u2010time results, which are crucial for in\u2010line monitoring of downstream processing, improving process development, control and optimization.<\/jats:p>","DOI":"10.1002\/biot.201700031","type":"journal-article","created":{"date-parts":[[2017,5,17]],"date-time":"2017-05-17T05:36:42Z","timestamp":1494999402000},"source":"Crossref","is-referenced-by-count":27,"title":["Universal label\u2010free in\u2010process quantification of influenza virus\u2010like particles"],"prefix":"10.1002","volume":"12","author":[{"given":"Sofia B.","family":"Carvalho","sequence":"first","affiliation":[{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica  Oeiras Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier Universidade Nova de Lisboa  Oeiras Portugal"}]},{"given":"Mafalda G.","family":"Moleirinho","sequence":"additional","affiliation":[{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica  Oeiras Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier Universidade Nova de Lisboa  Oeiras Portugal"}]},{"given":"David","family":"Wheatley","sequence":"additional","affiliation":[{"name":"Pall Life Sciences  Portsmouth UK"}]},{"given":"John","family":"Welsh","sequence":"additional","affiliation":[{"name":"Pall Life Sciences  Portsmouth UK"}]},{"given":"Ren\u00e9","family":"Gantier","sequence":"additional","affiliation":[{"name":"Pall Life Sciences  Westborough MA USA"}]},{"given":"Paula M.","family":"Alves","sequence":"additional","affiliation":[{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica  Oeiras Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier Universidade Nova de Lisboa  Oeiras Portugal"}]},{"given":"Cristina","family":"Peixoto","sequence":"additional","affiliation":[{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica  Oeiras Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier Universidade Nova de Lisboa  Oeiras Portugal"}]},{"given":"Manuel J. T.","family":"Carrondo","sequence":"additional","affiliation":[{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica  Oeiras Portugal"},{"name":"Departamento de Qu\u00edmica Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa  Monte da Caparica Portugal"}]}],"member":"311","published-online":{"date-parts":[[2017,7,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2613"},{"key":"e_1_2_7_3_1","unstructured":"World Health Organization Influenza (Seasonal)Fact Sheet No.211 March 2014.2014."},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2009.05.010"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3665"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3346"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1530"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2011.12.003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201400393"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2016.02.002"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/504831"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/wnan.119"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.2015.1046440"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2013.09.002"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2009.04.005"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.03.009"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/14760584.2016.1175942"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201400392"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-10-141"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.07.104"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055428"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0109616"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.08.077"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.02.090"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.11.027"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.10.070"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12372"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.02.049"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.2144\/03342bm05"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2009.03.007"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.6b00372"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-0346-0279-2_9"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13443"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-2659.2012.00375.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051559"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12896-015-0152-x"}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiot.201700031","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.201700031","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T13:39:37Z","timestamp":1759930777000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.201700031"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,5]]},"references-count":35,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1002\/biot.201700031"],"URL":"https:\/\/doi.org\/10.1002\/biot.201700031","archive":["Portico"],"relation":{},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"type":"print","value":"1860-6768"},{"type":"electronic","value":"1860-7314"}],"subject":[],"published":{"date-parts":[[2017,7,5]]},"article-number":"1700031"}}