{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T05:53:15Z","timestamp":1777528395231,"version":"3.51.4"},"reference-count":52,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,9,25]],"date-time":"2017-09-25T00:00:00Z","timestamp":1506297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/CTM\u2010BIO\/4388\/2014"],"award-info":[{"award-number":["PTDC\/CTM\u2010BIO\/4388\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BPD\/73322\/2010"],"award-info":[{"award-number":["SFRH\/BPD\/73322\/2010"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011198","name":"FP7 Research Potential of Convergence Regions","doi-asserted-by":"publisher","award":["FP7\u2010REGPOT\u20102012\u20102013\u20101"],"award-info":[{"award-number":["FP7\u2010REGPOT\u20102012\u20102013\u20101"]}],"id":[{"id":"10.13039\/100011198","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:sec><jats:label\/><jats:p>Many efforts are being directed worldwide to the treatment of OA\u2010focal lesions. The majority of those efforts comprise either the refinement of surgical techniques or combinations of biomaterials with various autologous cells. Herein, we tested electrospun polycaprolactone (PCL) nanofibrous meshes for cartilage tissue engineering. For that, articular chondrocytes (hACs) isolated from human osteoarthritic joints and Wharton's Jelly Stem Cells (hWJSCs) are cultured on electrospun nanofiber meshes, without adding external growth factors. We observed higher glycosaminoglycans production and higher over\u2010expression of cartilage\u2010related genes from hWJSCs cultured with basal medium, when compared to hACs isolated from osteoarthritic joints. Moreover, the presence of sulfated proteoglycans and collagen type II is observed on both types of cell cultures. We believe that this effect is due to either the electrospun nanofibers topography or the intrinsic chondrogenic differentiation potential of hWJSCs. Therefore, we propose the electrospun nanofibrous scaffolds in combination with hWJSCs as a viable alternative to the commercial membranes used in autologous chondrogenic regeneration approaches.<\/jats:p><\/jats:sec>","DOI":"10.1002\/biot.201700073","type":"journal-article","created":{"date-parts":[[2017,9,13]],"date-time":"2017-09-13T09:02:50Z","timestamp":1505293370000},"source":"Crossref","is-referenced-by-count":20,"title":["Electrospun Nanofibrous Meshes Cultured With Wharton's Jelly Stem Cell: An Alternative for Cartilage Regeneration, Without the Need of Growth Factors"],"prefix":"10.1002","volume":"12","author":[{"given":"Marta","family":"Alves da Silva","sequence":"first","affiliation":[{"name":"3B's Research Group \u2212 Biomaterials, Biodegradables and Biomimetics Dept. of Polymer Engineering University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark 4806\u2010909 Taipas Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's Laborat\u00f3rio Associado PT Government Associate Laboratory Portugal"}]},{"given":"Albino","family":"Martins","sequence":"additional","affiliation":[{"name":"3B's Research Group \u2212 Biomaterials, Biodegradables and Biomimetics Dept. of Polymer Engineering University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark 4806\u2010909 Taipas Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's Laborat\u00f3rio Associado PT Government Associate Laboratory Portugal"}]},{"given":"Ana R.","family":"Costa\u2010Pinto","sequence":"additional","affiliation":[{"name":"3B's Research Group \u2212 Biomaterials, Biodegradables and Biomimetics Dept. of Polymer Engineering University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark 4806\u2010909 Taipas Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's Laborat\u00f3rio Associado PT Government Associate Laboratory Portugal"}]},{"given":"N\u00e9lson","family":"Monteiro","sequence":"additional","affiliation":[{"name":"3B's Research Group \u2212 Biomaterials, Biodegradables and Biomimetics Dept. of Polymer Engineering University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark 4806\u2010909 Taipas Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's Laborat\u00f3rio Associado PT Government Associate Laboratory Portugal"}]},{"given":"Susana","family":"Faria","sequence":"additional","affiliation":[{"name":"Prof. S. Faria Department of Mathematics for Science and Technology, Research CMAT University of Minho Guimaraes Portugal"}]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[{"name":"3B's Research Group \u2212 Biomaterials, Biodegradables and Biomimetics Dept. of Polymer Engineering University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark 4806\u2010909 Taipas Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's Laborat\u00f3rio Associado PT Government Associate Laboratory Portugal"}]},{"given":"Nuno M.","family":"Neves","sequence":"additional","affiliation":[{"name":"3B's Research Group \u2212 Biomaterials, Biodegradables and Biomimetics Dept. of Polymer Engineering University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark 4806\u2010909 Taipas Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's Laborat\u00f3rio Associado PT Government Associate Laboratory Portugal"}]}],"member":"311","published-online":{"date-parts":[[2017,9,25]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.534"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2572"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-200110001-00033"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2008.01.042"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199410063311401"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1445-2197.2010.05495.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00167-004-0535-3"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aanat.2009.05.003"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.knee.2006.02.012"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.20-6-530"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2008.0393"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-004-1012-5"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2005.09.002"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.06.013"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368909X478560"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0197"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2004-0166"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2004-0013"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0709"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0002"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/7\/3\/035009"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2016.09.044"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658-016-0016-y"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0168059"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.36054"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007502828372"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2013.0301"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.2217\/17435889.2.6.929"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1179\/174328008X353547"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201200382"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2007.0327"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.2217\/nnm.10.31"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.1812"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm100476r"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.04.010"},{"key":"e_1_2_8_36_1","volume-title":"Biopolymer Methods in Tissue Engineering","author":"Kafienah W.","year":"2004"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34736"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm060680j"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.05.022"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/10\/1\/015018"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201200169"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-5527-z"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.200801648"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.08.054"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.04.075"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn402118s"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms140611692"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009016"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2008.00221.x"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-012-9418-z"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-011-9289-8"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.2174\/1574888X11308010012"}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiot.201700073","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.201700073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T13:36:26Z","timestamp":1759930586000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.201700073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,25]]},"references-count":52,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1002\/biot.201700073"],"URL":"https:\/\/doi.org\/10.1002\/biot.201700073","archive":["Portico"],"relation":{},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"value":"1860-6768","type":"print"},{"value":"1860-7314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,25]]},"article-number":"1700073"}}