{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:07:43Z","timestamp":1775606863148,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,10,6]],"date-time":"2017-10-06T00:00:00Z","timestamp":1507248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011102","name":"Seventh Framework Programme","doi-asserted-by":"publisher","award":["agreement Nr.312004"],"award-info":[{"award-number":["agreement Nr.312004"]}],"id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["project PTDC\/QEQ\u2010PRS\/0286\/2014; research unit iBB"],"award-info":[{"award-number":["project PTDC\/QEQ\u2010PRS\/0286\/2014; research unit iBB"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:sec><jats:label\/><jats:p>Monoclonal antibodies currently dominate the biopharmaceutical market with growing sales having reached 80 billion USD in 2016. As most top\u2010selling mAbs are approaching the end of their patent life, biopharmaceutical companies compete fiercely in the biosimilars market. These two factors present a strong motivation for alternative process strategies and process optimization. In this work a novel purification strategy for monoclonal antibodies comprising phenylboronic acid multimodal chromatography for capture followed by polishing by ion\u2010exchange monolithic chromatography and packed bed hydrophobic interaction chromatography is presented and compared to the traditional protein\u2010A\u2010based process. Although the capital investment is similar for both processes, the operation cost is 20% lower for the novel strategy. This study shows that the new process is worthwhile investing in and could present a viable alternative to the platform process used by most industrial players.<\/jats:p><\/jats:sec>","DOI":"10.1002\/biot.201700260","type":"journal-article","created":{"date-parts":[[2017,9,13]],"date-time":"2017-09-13T08:12:39Z","timestamp":1505290359000},"source":"Crossref","is-referenced-by-count":28,"title":["Monoclonal Antibodies Production Platforms: An Opportunity Study of a Non\u2010Protein\u2010A Chromatographic Platform Based on Process Economics"],"prefix":"10.1002","volume":"12","author":[{"given":"Ant\u00f3nio L.","family":"Grilo","sequence":"first","affiliation":[{"name":"iBB \u2013 Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior T\u00e9cnico Universidade de Lisboa Av. Rovisco Pais, No. 1 Lisbon 1049\u2010001 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2235-3988","authenticated-orcid":false,"given":"Mar\u00edlia","family":"Mateus","sequence":"additional","affiliation":[{"name":"iBB \u2013 Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior T\u00e9cnico Universidade de Lisboa Av. Rovisco Pais, No. 1 Lisbon 1049\u2010001 Portugal"}]},{"given":"Maria R.","family":"Aires\u2010Barros","sequence":"additional","affiliation":[{"name":"iBB \u2013 Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior T\u00e9cnico Universidade de Lisboa Av. Rovisco Pais, No. 1 Lisbon 1049\u2010001 Portugal"}]},{"given":"Ana M.","family":"Azevedo","sequence":"additional","affiliation":[{"name":"iBB \u2013 Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior T\u00e9cnico Universidade de Lisboa Av. Rovisco Pais, No. 1 Lisbon 1049\u2010001 Portugal"}]}],"member":"311","published-online":{"date-parts":[[2017,10,6]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1109-987"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1110-1165"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2060"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2437"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2794"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4161\/mabs.1.2.7736"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.24608"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coche.2015.07.005"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ces.2015.10.029"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201500354"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.4161\/mabs.2.5.12645"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.4161\/mabs.1.5.9448"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470444894.ch1"},{"key":"e_1_2_9_14_1","first-page":"427","volume-title":"Process Chemistry in the Pharmaceutical Industry","author":"Casnocha S.","year":"2007"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/10_014"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-1257-7_2"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1201\/9780849351068.ch12"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470444894.ch12"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-1257-7_14"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp070117s"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.1567"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470444894.ch2"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.10.034"},{"key":"e_1_2_9_24_1","first-page":"44","volume":"11","author":"Gagnon P.","year":"2013","journal-title":"BioProcess Int"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2009.01.004"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201400532"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.12665\/J74.Ward"},{"key":"e_1_2_9_28_1","author":"Oehme F.","year":"2010","journal-title":"Biopharm. Int"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.06.001"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2016.01.002"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201200328"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2014.06.005"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-012-6568-4"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.24902"},{"key":"e_1_2_9_35_1","first-page":"463","volume-title":"Process Scale Bioseparations for the Biopharmaceutical Industry","author":"Ghose S.","year":"2007"},{"key":"e_1_2_9_36_1","first-page":"179","volume-title":"Process Scale Bioseparations for the Biopharmaceutical Industry","author":"Shukla A. A.","year":"2007"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.24539"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.4161\/19420862.2015.989042"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/mde.1360"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/9783527669417.ch22"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cep.2005.03.006"},{"key":"e_1_2_9_42_1","volume-title":"Plant Design and Economics for Chemical Engineers","author":"Peters M. S.","year":"2003"},{"key":"e_1_2_9_43_1","first-page":"1227","volume-title":"Intellectual Property Management in Health and Agriculture Innovation: A Handbook of Best Practices","author":"Edwards M. G.","year":"2007"}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiot.201700260","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.201700260","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T13:36:31Z","timestamp":1759930591000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.201700260"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,6]]},"references-count":43,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1002\/biot.201700260"],"URL":"https:\/\/doi.org\/10.1002\/biot.201700260","archive":["Portico"],"relation":{},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"value":"1860-6768","type":"print"},{"value":"1860-7314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,6]]},"article-number":"1700260"}}