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Scalable expansion of hiPSC is often performed using polystyrene microcarriers, which have to be removed from the cell suspension using a separation step that causes loss of viable cells. In this study, application of novel xeno\u2010free dissolvable microcarriers (DM) for an efficient and integrated expansion and harvesting of hiPSC is demonstrated. After an initial screening under static conditions, hiPSC culture using DM is performed in dynamic culture, using spinner\u2010flasks. A maximum 4.0\u2009\u00b1\u20090.8\u2010fold expansion is achieved after 5 days of culture. These results are validated with a second cell line and the culture is successfully adapted to fully xeno\u2010free conditions. Afterwards, cell recovery is made within the spinner flask, being obtained a 92\u2009\u00b1\u20094% harvesting yield, which is significantly higher than the one obtained for the conventional filtration\u2010based method (45\u2009\u00b1\u20093%). Importantly, the expanded and harvested hiPSC maintain their pluripotency and multilineage differentiation potential. The results here described represent a significant improvement of the downstream processing after microcarrier\u2010based hiPSC expansion, leading to a more cost\u2010effective and efficient bioprocess.<\/jats:p><\/jats:sec>","DOI":"10.1002\/biot.201800461","type":"journal-article","created":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T05:06:50Z","timestamp":1539580010000},"source":"Crossref","is-referenced-by-count":65,"title":["Dissolvable Microcarriers Allow Scalable Expansion And Harvesting Of Human Induced Pluripotent Stem Cells Under Xeno\u2010Free Conditions"],"prefix":"10.1002","volume":"14","author":[{"given":"Andr\u00e9 L.","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Department of Bioengineering and iBB\u2010Institute for Bioengineering and Biosciences Instituto Superior T\u00e9cnico Universidade de Lisboa 1049\u2010001 Lisboa Portugal"},{"name":"The Discoveries Centre for Regenerative and Precision Medicine Lisbon Campus Instituto Superior T\u00e9cnico Universidade de Lisboa 1049\u2010001 Lisboa Portugal"}]},{"given":"Carlos A. V.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and iBB\u2010Institute for Bioengineering and Biosciences Instituto Superior T\u00e9cnico Universidade de Lisboa 1049\u2010001 Lisboa Portugal"},{"name":"The Discoveries Centre for Regenerative and Precision Medicine Lisbon Campus Instituto Superior T\u00e9cnico Universidade de Lisboa 1049\u2010001 Lisboa Portugal"}]},{"given":"Ana R.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and iBB\u2010Institute for Bioengineering and Biosciences Instituto Superior T\u00e9cnico Universidade de Lisboa 1049\u2010001 Lisboa Portugal"},{"name":"The Discoveries Centre for Regenerative and Precision Medicine Lisbon Campus Instituto Superior T\u00e9cnico Universidade de Lisboa 1049\u2010001 Lisboa Portugal"}]},{"given":"Sara F.","family":"Vieira","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and iBB\u2010Institute for Bioengineering and Biosciences Instituto Superior T\u00e9cnico Universidade de Lisboa 1049\u2010001 Lisboa Portugal"}]},{"given":"Sara M.","family":"Badenes","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and iBB\u2010Institute for Bioengineering and Biosciences Instituto Superior T\u00e9cnico Universidade de Lisboa 1049\u2010001 Lisboa Portugal"}]},{"given":"Maria M.","family":"Diogo","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and iBB\u2010Institute for Bioengineering and Biosciences Instituto Superior T\u00e9cnico Universidade de Lisboa 1049\u2010001 Lisboa Portugal"},{"name":"The Discoveries Centre for Regenerative and Precision Medicine Lisbon Campus Instituto Superior T\u00e9cnico Universidade de Lisboa 1049\u2010001 Lisboa Portugal"}]},{"given":"Joaquim M.S.","family":"Cabral","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and iBB\u2010Institute for Bioengineering and Biosciences Instituto Superior T\u00e9cnico Universidade de Lisboa 1049\u2010001 Lisboa Portugal"},{"name":"The Discoveries Centre for Regenerative and Precision Medicine Lisbon Campus Instituto Superior T\u00e9cnico Universidade de Lisboa 1049\u2010001 Lisboa Portugal"}]}],"member":"311","published-online":{"date-parts":[[2018,11,12]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.11.019"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2015.27"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2012.03.003"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.4189"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2008.0262"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2011.06.009"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2016.07.023"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2015-0253"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023212"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2009.02.005"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2012.0146"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0151264"},{"key":"e_1_2_8_13_1","author":"Rodrigues C. 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