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Previous works attempted the biological production of the full WW domain in tandem to generate multivalent components for affinity\u2010triggered hydrogels. In this work, an alternative approach is followed by engineering a 13\u2010mer minimal version of the WW domain that retains the ability to bind to target proline\u2010rich peptides. Both ligand and target peptides are produced chemically and conjugated to multivalent polyethylene glycol, yielding two components. Upon mixing together, they form soft biocompatible affinity\u2010triggered assemblies, stable in stem cell culture media, and display mechanical properties in the same order of magnitude as for those hydrogels formed with the full WW protein in tandem.<\/jats:p>","DOI":"10.1002\/biot.201800559","type":"journal-article","created":{"date-parts":[[2019,7,8]],"date-time":"2019-07-08T08:29:22Z","timestamp":1562574562000},"source":"Crossref","is-referenced-by-count":3,"title":["Affinity\u2010Triggered Assemblies Based on a Designed Peptide\u2013Peptide Affinity Pair"],"prefix":"10.1002","volume":"14","author":[{"given":"Cl\u00e1udia S. 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