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This constant reformulation adds several challenges to manufacturing, particularly in purification due to the variation of the physicochemical properties of the vaccine product. A universal platform approach capable of handling such variation is therefore of utmost importance. In this work, a filtration\u2010based approach is explored to purify influenza virus\u2010like particles. Switching from adsorptive separation to size\u2010based purification allows overcoming the differences in retention observed for different influenza strains. The proposed process employs a cascade of ultrafiltration and diafiltration steps, followed by a sterile filtration step. Different process parameters are assessed in terms of product recovery and impurities\u2019 removal. Membrane chemistry, pore size, operation modes, critical flux, transmembrane pressure, and permeate control strategies are evaluated. After membrane selection and parameter optimization, concentration factors and diafiltration volumes are also defined. By optimizing the filtration mode of operation, it is possible to achieve product recoveries of approximately 80%. Overall, the process time is decreased by 30%, its scalability is improved, and the costs are reduced due to the removal of chromatography and associated buffer consumptions, cleaning, and its validation steps.<\/jats:p>","DOI":"10.1002\/biot.201800570","type":"journal-article","created":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T07:22:52Z","timestamp":1558336972000},"source":"Crossref","is-referenced-by-count":39,"title":["Membrane\u2010Based Approach for the Downstream Processing of Influenza Virus\u2010Like Particles"],"prefix":"10.1002","volume":"14","author":[{"given":"Sofia B.","family":"Carvalho","sequence":"first","affiliation":[{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica Oeiras Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier Universidade Nova de Lisboa Av. da Rep\u00fablica 2780\u2010157 Oeiras Portugal"}]},{"given":"Ricardo J. 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