{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T21:02:11Z","timestamp":1762376531746,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T00:00:00Z","timestamp":1558310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/104498\/2014"],"award-info":[{"award-number":["SFRH\/BD\/104498\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:p>Protein A affinity chromatography is a core unit operation in antibody manufacturing. Nevertheless, there is not enough understanding of in\u2010column antibody adsorption in the Protein A capture step. This work aims to investigate in situ the establishment of an antibody (trastuzumab) layer during Protein A chromatography both in terms of energetic contributions and uptake kinetics. Flow microcalorimetry is employed as a technique with an in situ operating detector, which provides an understanding of the thermodynamics of the adsorption process. In addition, the antibody uptake rate is also investigated in order to establish a correlation between its diffusion on the stationary phase and the associated thermodynamics. Two resins with different particle size, intraparticle porosity, and a Protein A ligand structure are studied: the synthetically engineered B\u2010domain tetrameric MabSelect SuRe and the synthetically engineered C\u2010domain hexameric TOYOPEARL AF\u2010rProtein A HC. The uptake rate follows a pore diffusion model at low equilibrium time, showing a slower diffusivity after a certain time because of the heterogeneous binding nature of these two resins. In addition, the microcalorimetric studies show that adsorption enthalpy is highly favourable at low isotherm concentrations and evolves toward an equilibrium with increasing surface concentration. These data suggest that the relationship between adsorption enthalpy and the establishment of the antibody layer in the Protein A chain is consistent with heterogeneous adsorption.<\/jats:p>","DOI":"10.1002\/biot.201800632","type":"journal-article","created":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T04:20:56Z","timestamp":1554351656000},"source":"Crossref","is-referenced-by-count":13,"title":["Antibody Binding Heterogeneity of Protein A Resins"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8203-990X","authenticated-orcid":false,"given":"Gon\u00e7alo F. L.","family":"da Silva","sequence":"first","affiliation":[{"name":"CICS\u2010UBI, Health Sciences Research Centre University of Beira Interior  Av. Infante D. Henrique 6200\u2010506 Covilh\u00e3 Portugal"},{"name":"Department of Chemistry University of Beira Interior  Av. Marqu\u00eas d\u2019\u00c1vila e Bolama 6201\u2010001 Covilh\u00e3 Portugal"},{"name":"Austrian Centre of Industrial Biotechnology  Muthgasse 18 1190 Vienna Austria"}]},{"given":"Jacek","family":"Plewka","sequence":"additional","affiliation":[{"name":"Austrian Centre of Industrial Biotechnology  Muthgasse 18 1190 Vienna Austria"},{"name":"Department of Material Science and Process Engineering University of Natural Resources and Life Sciences Peter\u2010Jordan Strasse 82 1190 Vienna Austria"}]},{"given":"Rupert","family":"Tschelie\u00dfnig","sequence":"additional","affiliation":[{"name":"Austrian Centre of Industrial Biotechnology  Muthgasse 18 1190 Vienna Austria"},{"name":"Department of Biotechnology University of Natural Resources and Life Sciences Muthgasse 18 1190 Vienna Austria"}]},{"given":"Helga","family":"Lichtenegger","sequence":"additional","affiliation":[{"name":"Austrian Centre of Industrial Biotechnology  Muthgasse 18 1190 Vienna Austria"},{"name":"Department of Material Science and Process Engineering University of Natural Resources and Life Sciences Peter\u2010Jordan Strasse 82 1190 Vienna Austria"}]},{"given":"Alois","family":"Jungbauer","sequence":"additional","affiliation":[{"name":"Austrian Centre of Industrial Biotechnology  Muthgasse 18 1190 Vienna Austria"},{"name":"Department of Biotechnology University of Natural Resources and Life Sciences Muthgasse 18 1190 Vienna Austria"}]},{"given":"Ana C. M.","family":"Dias\u2010Cabral","sequence":"additional","affiliation":[{"name":"CICS\u2010UBI, Health Sciences Research Centre University of Beira Interior  Av. Infante D. Henrique 6200\u2010506 Covilh\u00e3 Portugal"},{"name":"Department of Chemistry University of Beira Interior  Av. Marqu\u00eas d\u2019\u00c1vila e Bolama 6201\u2010001 Covilh\u00e3 Portugal"}]}],"member":"311","published-online":{"date-parts":[[2019,5,20]]},"reference":[{"key":"e_1_2_7_1_1_1","doi-asserted-by":"publisher","DOI":"10.4161\/19420862.2015.989042"},{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3917"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.09.026"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.09.030"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-123011-1.50006-X"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)43463-6"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5456.1279"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.04.035"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21044"},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2016.06.031"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.20729"},{"key":"e_1_2_7_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0298-190"},{"key":"e_1_2_7_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2015.03.080"},{"key":"e_1_2_7_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2015.08.068"},{"key":"e_1_2_7_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmr.564"},{"key":"e_1_2_7_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.05.033"},{"key":"e_1_2_7_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.05.049"},{"key":"e_1_2_7_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.11.002"},{"key":"e_1_2_7_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.07.050"},{"key":"e_1_2_7_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.09.010"},{"key":"e_1_2_7_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.07.050"},{"key":"e_1_2_7_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.09.017"},{"key":"e_1_2_7_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2016.01.036"},{"key":"e_1_2_7_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.03.060"},{"key":"e_1_2_7_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0032-9592(93)80023-A"},{"key":"e_1_2_7_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(03)00092-8"},{"key":"e_1_2_7_1_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1989.tb15244.x"},{"key":"e_1_2_7_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/9783527630158.ch6"},{"key":"e_1_2_7_1_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/i200032a003"},{"key":"e_1_2_7_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.05.051"},{"key":"e_1_2_7_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.12.058"},{"key":"e_1_2_7_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.26843"},{"key":"e_1_2_7_1_33_1","first-page":"p. 63","volume-title":"Adsorption Engineering","author":"Suzuki M.","year":"1990"},{"key":"e_1_2_7_1_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/01932699208943322"},{"key":"e_1_2_7_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.04.009"}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiot.201800632","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.201800632","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/biot.201800632","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.201800632","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T11:45:20Z","timestamp":1759923920000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.201800632"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,20]]},"references-count":35,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2019,8]]}},"alternative-id":["10.1002\/biot.201800632"],"URL":"https:\/\/doi.org\/10.1002\/biot.201800632","archive":["Portico"],"relation":{},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"type":"print","value":"1860-6768"},{"type":"electronic","value":"1860-7314"}],"subject":[],"published":{"date-parts":[[2019,5,20]]},"article-number":"1800632"}}