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Bioreactors that provide different biophysical stimuli have been used in tissue engineering approaches aimed at enhancing the quality of the cartilage tissue generated. However, such systems are often highly complex, expensive, and not very versatile. In the current study, a novel, cost\u2010effective, and customizable perfusion bioreactor totally fabricated by additive manufacturing (AM) is proposed for the study of the effect of fluid flow on the chondrogenic differentiation of human bone\u2010marrow mesenchymal stem\/stromal cells (hBMSCs) in 3D porous poly(\u025b\u2010caprolactone) (PCL) scaffolds. hBMSCs are first seeded and grown on PCL scaffolds and hBMSC\u2013PCL constructs are then transferred to 3D\u2010extruded bioreactors for continuous perfusion culture under chondrogenic inductive conditions. Perfused constructs show similar cell metabolic activity and significantly higher sulfated glycosaminoglycan production (\u22481.8\u2010fold) in comparison to their non\u2010perfused counterparts. Importantly, perfusion bioreactor culture significantly promoted the expression of chondrogenic marker genes while downregulating hypertrophy. This work highlights the potential of customizable AM platforms for the development of novel personalized repair strategies and more reliable in vitro models with a wide range of applications.<\/jats:p>","DOI":"10.1002\/biot.201900078","type":"journal-article","created":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T06:06:50Z","timestamp":1569564410000},"source":"Crossref","is-referenced-by-count":11,"title":["Extruded Bioreactor Perfusion Culture Supports the Chondrogenic Differentiation of Human Mesenchymal Stem\/Stromal Cells in 3D Porous Poly(\u025b\u2010Caprolactone) Scaffolds"],"prefix":"10.1002","volume":"15","author":[{"given":"Jo\u00e3o C.","family":"Silva","sequence":"first","affiliation":[{"name":"Department of Bioengineering and iBB \u2010 Institute of Bioengineering and Biosciences Instituto Superior T\u00e9cnico Universidade de Lisboa  Av. Rovisco Pais Lisboa 1049\u2010001 Portugal"},{"name":"Department of Chemistry and Chemical Biology, Biological Sciences, Biomedical Engineering and Chemical and Biological Engineering Center for Biotechnology and Interdisciplinary Studies Rensselaer Polytechnic Institute  Troy NY 12180\u20103590 USA"}]},{"given":"Carla S.","family":"Moura","sequence":"additional","affiliation":[{"name":"CDRSP \u2013 Centre for Rapid and Sustainable Product Development Polytechnic Institute of Leiria  Rua de Portugal\u2010Zona Industrial Marinha Grande 2430\u2010028 Portugal"}]},{"given":"Gon\u00e7alo","family":"Borrecho","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o Interdisciplinar Egas Moniz (CiiEM) Quinta da Granja  Monte da Caparica 2829\u2010511 Caparica Portugal"}]},{"given":"Ant\u00f3nio P. 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