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Precipitation and magnetic separation are two techniques already proven to accomplish protein purification from complex media, yet never used in synergy. With the aim to capture antibodies directly from crude extracts, a new approach combining precipitation and magnetic separation is developed and named as affinity magnetic precipitation. A precipitation screening, based on the Hofmeister series, and a commercial precipitation kit are tested with affinity magnetic particles to assess the best condition for antibody capture from human serum plasma and clarified cell supernatant. The best conditions are obtained when using PEG3350 as precipitant at 4\u00a0\u00b0C for 1\u00a0h, reaching 80% purity and 50% recovery of polyclonal antibodies from plasma, and 99% purity with 97% recovery yield of anti\u2010TNF\u03b1 mAb from cell supernatants. These results show that the synergetic use of precipitation and magnetic separation can represent an alternative for the efficient capture of antibodies.<\/jats:p>","DOI":"10.1002\/biot.202000151","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T06:48:05Z","timestamp":1592981285000},"source":"Crossref","is-referenced-by-count":6,"title":["Magnetic Precipitation: A New Platform for Protein Purification"],"prefix":"10.1002","volume":"15","author":[{"given":"Raquel dos","family":"Santos","sequence":"first","affiliation":[{"name":"UCIBIO Chemistry Department NOVA School of Science and Technology  Caparica 2829\u2010516 Portugal"}]},{"given":"In\u00eas","family":"Iria","sequence":"additional","affiliation":[{"name":"iMed.ULisboa \u2010 Research Institute for Medicines, Faculty of Pharmacy University of Lisbon, Avenida Prof. Gama Pinto  Lisbon 1649\u2010003 Portugal"}]},{"given":"Ana M.","family":"Manuel","sequence":"additional","affiliation":[{"name":"iMed.ULisboa \u2010 Research Institute for Medicines, Faculty of Pharmacy University of Lisbon, Avenida Prof. Gama Pinto  Lisbon 1649\u2010003 Portugal"}]},{"given":"Ana P.","family":"Leandro","sequence":"additional","affiliation":[{"name":"iMed.ULisboa \u2010 Research Institute for Medicines, Faculty of Pharmacy University of Lisbon, Avenida Prof. Gama Pinto  Lisbon 1649\u2010003 Portugal"}]},{"given":"Catarina A. 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