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Our findings demonstrated that several compounds exhibit remarkable effects, enhancing the long\u2010term storage of plasmid DNA at room temperature and the resilience of RNA to high\u2010temperature stress, surpassing the performance of commercial osmolytes. Importantly, we found that one of the compounds enhanced the detection efficacy of a cost\u2010effective RT\u2010PCR test for COVID\u201019 that we had previously developed.<\/jats:p><jats:p>This work offers new possibilities for expanding the capabilities of molecular diagnostic assays and nucleic acid storage methods.<\/jats:p>","DOI":"10.1002\/biot.202400608","type":"journal-article","created":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T16:51:16Z","timestamp":1741625476000},"source":"Crossref","is-referenced-by-count":2,"title":["Synthetic Osmolytes for Enhanced RNA Thermotolerance and Extended Plasmid Storage at Room Temperature"],"prefix":"10.1002","volume":"20","author":[{"given":"Catarina","family":"Amaral","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier Universidade Nova de Lisboa  Oeiras Portugal"}]},{"given":"Eva C.","family":"Louren\u00e7o","sequence":"additional","affiliation":[{"name":"Extremochem Lda  Lisboa Portugal"}]},{"given":"In\u00eas","family":"Morais","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier Universidade Nova de Lisboa  Oeiras Portugal"}]},{"given":"Osvaldo","family":"Ascenso","sequence":"additional","affiliation":[{"name":"Extremochem Lda  Lisboa Portugal"}]},{"given":"Sandra I.","family":"Aguiar","sequence":"additional","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health (CIISA) Faculty of Veterinary Medicine Universidade de Lisboa  Lisboa Portugal"}]},{"given":"Diana","family":"Grilo","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier Universidade Nova de Lisboa  Oeiras Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6854-7278","authenticated-orcid":false,"given":"M. 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