{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T09:41:51Z","timestamp":1698313311694},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"vor","delay-in-days":3593,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biopolymers"],"published-print":{"date-parts":[[1994,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aggregation of filipin in aqueous medium and filipin\u2010induced changes in cholesterol micelles have been studied using intensity and dynamic light scattering. The dependencies of filipin aggregate dimensions on concentration, solvent, and temperature were studied, and revealed that the aggregates do not have a well\u2010defined geometry, i.e., a critical micelle concentration cannot be detected and stable structures are not formed. The aggregates are of size <jats:italic>R<\/jats:italic><jats:sub>g<\/jats:sub> \u2248 110 nm and <jats:italic>R<\/jats:italic><jats:sub>h<\/jats:sub> \u2248 63 nm, referring to the radius of gyration and hydrodynamic radius, respectively. In the concentration range studied (1 \u03bc<jats:italic>M<\/jats:italic> &lt; <jats:italic>C<\/jats:italic> &lt; 30 \u03bc<jats:italic>M<\/jats:italic>), a low molecular weight species (monomer\/dimer) is always present together with the aggregates. In ethanol\/ water mixtures, large (<jats:italic>R<\/jats:italic><jats:sub>g<\/jats:sub> \u2248 500 nm), narrow distribution aggregates are formed in the water volume fraction range 0.45 &lt; \u03a6<jats:sub>H<\/jats:sub>2<jats:sub>O<\/jats:sub> &lt; 0.65. Aggregation also occurs on changing the temperature; In the range 7\u201337\u00b0C, smaller aggregates (10\u201330 nm) form and the process is only partially reversible.<\/jats:p><jats:p>No pronounced effect of filipin on the structure of the cholesterol micelles was observed (a small increase in R<jats:sub>g<\/jats:sub> and R<jats:sub>h<\/jats:sub> is noted). These results rule out any \u201cspecificity\u201d for the filipin interactions with cholesterol, which has been considered a key event in the filipin biochemical mode of action. A reevaluatiori Of this question is suggested and some alternatives are advanced. \u00a9 1994 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/bip.360340402","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T11:34:52Z","timestamp":1104320092000},"page":"447-456","source":"Crossref","is-referenced-by-count":4,"title":["Filipin and its interaction with cholesterol in aqueous media studied using static and dynamic light scattering"],"prefix":"10.1002","volume":"34","author":[{"given":"Miguel A. R. B.","family":"Castanho","sequence":"first","affiliation":[]},{"given":"Wyn","family":"Brown","sequence":"additional","affiliation":[]},{"given":"Manuel J. E.","family":"Prieto","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,2]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1128\/jb.80.2.200-206.1960","volume":"80","author":"Lampen J. 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