{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T07:51:11Z","timestamp":1768895471477,"version":"3.49.0"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,5,15]],"date-time":"2006-05-15T00:00:00Z","timestamp":1147651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[2006,9,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this work the question was addressed if in nitrite\u2010oxidizing activated sludge systems the environmental competition between <jats:italic>Nitrobacter<\/jats:italic> spp. and <jats:italic>Nitrospira<\/jats:italic> spp., which only recently has been discovered to play a role in these systems, is affected by the nitrite concentrations. Two parallel chemostats were inoculated with nitrifying\u2010activated sludge containing <jats:italic>Nitrospira<\/jats:italic> and operated under identical conditions. After addition of <jats:italic>Nitrobacter<\/jats:italic> to both chemostats, the nitrite concentration in the influent of one of the chemostats was increased such that nitrite peaks in the bulk liquid of this reactor were detected. The other chemostat served as control reactor, which always had a constant nitrite influent concentration. The relative cellular area (RCA) of <jats:italic>Nitrospira<\/jats:italic> and <jats:italic>Nitrobacter<\/jats:italic> was determined by quantitative fluorescence in situ hybridization (FISH). The nitrite perturbation stimulated the growth of <jats:italic>Nitrobacter<\/jats:italic> while in the undisturbed control chemostat <jats:italic>Nitrospira<\/jats:italic> dominated. Overall, the results of this experimental study support the hypothesis that <jats:italic>Nitrobacter<\/jats:italic> is a superior competitor when resources are abundant, while <jats:italic>Nitrospira<\/jats:italic> thrive under conditions of resource scarcity. Interestingly, the dominance of <jats:italic>Nitrobacter<\/jats:italic> over <jats:italic>Nitrospira<\/jats:italic>, caused by the elevated nitrite concentrations, could not be reverted by lowering the available nitrite concentration to the original level. One possible explanation for this result is that when <jats:italic>Nitrobacter<\/jats:italic> is present at a certain cell density it is able to inhibit the growth of <jats:italic>Nitrospira.<\/jats:italic> An alternative explanation would be that the length of the experimental period was not long enough to observe an increase of the <jats:italic>Nitrospira<\/jats:italic> population. \u00a9 2006 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.21004","type":"journal-article","created":{"date-parts":[[2006,5,15]],"date-time":"2006-05-15T19:55:25Z","timestamp":1147722925000},"page":"169-175","source":"Crossref","is-referenced-by-count":121,"title":["Competition between <i>Nitrospira<\/i> spp. and <i>Nitrobacter<\/i> spp. in nitrite\u2010oxidizing bioreactors"],"prefix":"10.1002","volume":"95","author":[{"given":"Regina","family":"Nogueira","sequence":"first","affiliation":[]},{"given":"Lu\u00eds F.","family":"Melo","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,5,15]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.2.762-770.1990"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-0611-6_3"},{"key":"e_1_2_1_4_1","volume-title":"American public health association","author":"APHA, Standard Methods for the Examination of Water and Wastewater","year":"1995"},{"key":"e_1_2_1_5_1","first-page":"83","volume-title":"Microbial ecology: Fundamentals and applications","author":"Atlas RM","year":"1993"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"4126","DOI":"10.1128\/AEM.65.9.4126-4133.1999","article-title":"Identification of nitrite\u2010oxidizing bacteria with monoclonal antibodies recognizing nitrite oxidoreductase","volume":"65","author":"Bartosch S","year":"1999","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0043-1354(92)90146-U"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0290(20001220)70:6<677::AID-BIT10>3.0.CO;2-3"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(99)80053-8"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.11.5273-5284.2001"},{"key":"e_1_2_1_11_1","first-page":"55","volume-title":"Wastewater treatment: Biological and chemical processes","author":"Henze M","year":"1995"},{"key":"e_1_2_1_12_1","first-page":"195","volume-title":"Wastewater treatment: Biological and chemical processes","author":"Jansen JC","year":"1995"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"3042","DOI":"10.1128\/AEM.64.8.3042-3051.1998","article-title":"Combined molecular and conventional analyses of nitrifying bacterium in activated sludge: Nitrosococcus mobilis and Nitrospira\u2010like bacteria as dominant populations","volume":"64","author":"Juretschko S","year":"1998","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2005.12.023"},{"key":"e_1_2_1_15_1","first-page":"42","volume-title":"Experimental microbial ecology","author":"Kuenen JG","year":"1982"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(11)80121-9"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0043-1354(76)90022-1"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(00)80050-8"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.8.3690-3696.1999"},{"key":"e_1_2_1_20_1","unstructured":"StrousM.2000.Microbiology and application of anaerobic ammonium oxidation. 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