{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:24:06Z","timestamp":1760707446059,"version":"build-2065373602"},"reference-count":10,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2004,2,18]],"date-time":"2004-02-18T00:00:00Z","timestamp":1077062400000},"content-version":"vor","delay-in-days":5249,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[1989,10,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A novel means of extracting carboxylic acids from aqueous solutions is described which involves the use of a lipolytic enzyme to convert the acid to a more hydrophobic ester. A water\u2010immiscible long\u2010chain alcohol serves both as a reactant in esterifying the acid, and as a solvent for extraction of the ester. Radiochemical tracer studies of the underlying reaction and extraction equilibria established that a low equilibrium concentration of ester (0.04\u20130.5m<jats:italic>M<\/jats:italic>) in the presence of water is counterbalanced by the high distribution coefficient of the ester (220\u20133380, wt. basis). The net result is a substantial (4\u2010 to 15\u2010fold) increase in the apparent distribution coefficient of the acid; 80%\u201395% of the extracted acid is in the esterified form. The method is applicable to a variety of alcohols and acids.<\/jats:p>","DOI":"10.1002\/bit.260340705","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T23:17:49Z","timestamp":1104362269000},"page":"909-915","source":"Crossref","is-referenced-by-count":14,"title":["Esterification\u2010coupled extraction of organic acids: Partition enhancement and underlying reaction and distribution equilibria"],"prefix":"10.1002","volume":"34","author":[{"given":"M. R.","family":"Aires\u2010Barros","sequence":"first","affiliation":[]},{"given":"J. M. S.","family":"Cabral","sequence":"additional","affiliation":[]},{"given":"R. C.","family":"Willson","sequence":"additional","affiliation":[]},{"given":"J.\u2010F. P.","family":"Hamel","sequence":"additional","affiliation":[]},{"given":"C. L.","family":"Cooney","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,2,18]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"305","volume":"44","author":"Finn R. K.","year":"1966","journal-title":"J. Ferm. Technol."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7799(84)90022-2"},{"key":"e_1_2_1_4_2","first-page":"185","volume-title":"Biocatalysis in Organic Media","author":"Barros M. R. A.","year":"1987"},{"key":"e_1_2_1_5_2","first-page":"593","volume":"61","author":"Yongsmith B.","year":"1983","journal-title":"J. Ferm. Technol."},{"key":"e_1_2_1_6_2","first-page":"551","volume":"61","author":"Nanba A.","year":"1983","journal-title":"J. Ferm. Technol."},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260300112"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb1961.37.999"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1984.tb29892.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260280217"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.25.1980"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.260340705","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.260340705","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:08:34Z","timestamp":1760054914000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.260340705"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,10,5]]},"references-count":10,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1989,10,5]]}},"alternative-id":["10.1002\/bit.260340705"],"URL":"https:\/\/doi.org\/10.1002\/bit.260340705","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"type":"print","value":"0006-3592"},{"type":"electronic","value":"1097-0290"}],"subject":[],"published":{"date-parts":[[1989,10,5]]}}}