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Results obtained in continuous stirred\u2010tank reactors (CSTR) operated at pH values between 6.6 and 7.8 showed that growth inhibition depended only on the nitrite undissociated fraction concentration (nitrous acid). A mathematical model to describe this dependence is put forward. The maximum nitrous acid concentration compatible with cell growth and denitrification activity was found to be 66 \u03bcg N\/L. Denitrification activity was partially associated with growth, as described by the Luedeking\u2013Piret equation. However, when the freshly inoculated reactor was operated discontinuosly, nitrite accumulation caused growth uncoupling from denitrification activity. The authors suggest that these results can be interpreted considering that (a) nitrous acid acts as a proton uncoupler; and (b) cultures continuoulsy exposed to nitrous acid prevent the uncoupling effect but not the growth inhibition. Examination of the growth dependence on nitrite concentration at pH 7.0 showed that adapted cultures (grown on CSTR) are less sensitive to nitrous acid inhibition than the ones cultivated in batch. \u00a9 1995 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/bit.260460303","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T19:09:14Z","timestamp":1104347354000},"page":"194-201","source":"Crossref","is-referenced-by-count":154,"title":["Nitrite inhibition of denitrification by <i>Pseudomonas fluorescens<\/i>"],"prefix":"10.1002","volume":"46","author":[{"given":"J. S.","family":"Almeida","sequence":"first","affiliation":[]},{"given":"S. M.","family":"J\u00falio","sequence":"additional","affiliation":[]},{"given":"M. A. M.","family":"Reis","sequence":"additional","affiliation":[]},{"given":"M. J. 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