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Gallic, protocatechuic, <jats:italic>p<\/jats:italic>\u2010hydroxybenzoic, vanillic acids, apigenin 7\u2010<jats:italic>O<\/jats:italic>\u2010glucoside, luteolin 7\u2010<jats:italic>O<\/jats:italic>\u2010glucoside, and rutin were identified in olive leaves. The amount of oleuropein was equal to 22.64 g\/kg dry weight. (<jats:italic>E<\/jats:italic>)\u2010Anethole (32.35%), fenchone (11.89%), and (<jats:italic>Z<\/jats:italic>)\u20103\u2010nonen\u20101\u2010ol (8%) were found to be the main constituents of the oil volatile fraction, whereas palmitic, oleic, and alpha\u2010linolenic acid were determined to be dominating fatty acids. Olive leaves methanol extract was observed to exerted a significant, selective cytotoxic effect on DU\u2010145 and <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>\u20103 cell lines. Except the essential oil composition, evaluated wild olive leaves, with regard to their quantitative and qualitative composition, do not substantially differ from the leaves of other cultivars grown for industrial purposes and they reveal considerable antioxidant and cytotoxic properties. 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