{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T08:06:38Z","timestamp":1753862798650,"version":"3.41.2"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T00:00:00Z","timestamp":1650326400000},"content-version":"am","delay-in-days":350,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T00:00:00Z","timestamp":1620086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006136","name":"Fuel Cell Technologies Program","doi-asserted-by":"publisher","award":["CEECIND\/01120\/2017"],"award-info":[{"award-number":["CEECIND\/01120\/2017"]}],"id":[{"id":"10.13039\/100006136","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The lipid fraction of seeds from different pine species and populations was studied regarding total lipid content, fatty acid profile and vitamin E composition. The investigated seeds contained a high percentage of lipid (13.6 to 31.5\u2009%). Lipid fractions were found to be rich in vitamin E, which varied significantly among species and populations. <jats:italic>P. halepensis<\/jats:italic> (Ph\u2212Hn) showed the highest content of vitamin E (256.3\u2005mg\/kg of seeds) and the uppermost content of <jats:italic>\u03b1<\/jats:italic>\u2010tocopherol (44\u2005mg\/kg). However, <jats:italic>P. halepensis<\/jats:italic> (Ph\u2212Kas) was the richest in <jats:italic>\u03b3<\/jats:italic>\u2010tocopherol (204.9\u2005mg\/kg). Lipid fractions had a low content of <jats:italic>\u03b4<\/jats:italic>\u2010tocopherol (1.2 to 3.6\u2005mg\/kg. The highest content of <jats:italic>\u03b3<\/jats:italic>\u2010tocotrienol (\u223c18\u2009%) was determined for <jats:italic>P. halepensis<\/jats:italic> (Ph\u2212Dc and Ph\u2212Hn). Thirteen fatty acids were identified by GC\u2010FID with significant variation between the investigated species. The linoleic acid was the major fatty acid followed by oleic acid and palmitic acid. The chemical differentiation among species for the composition of fatty acids and vitamin E was confirmed by PCA. Significant correlations were observed between the content of vitamin E and fatty acids and ecological parameters of <jats:italic>P. halepensis<\/jats:italic> populations.<\/jats:p>","DOI":"10.1002\/cbdv.202100071","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T09:27:13Z","timestamp":1618824433000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Morphological and Chemical Differentiation between Tunisian Populations of <i>Pinus halepensis<\/i>, <i>Pinus brutia<\/i>, and <i>Pinus pinaster<\/i>"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6262-1000","authenticated-orcid":false,"given":"Mariem","family":"Khouja","sequence":"first","affiliation":[{"name":"National Institute of Applied Science and Technology Department of Biology Laboratory of Nanobiotechnology and Valorization of Medicinal Phytoresources University of Carthage  B.P. 676 1080 Tunis Cedex Tunisia"},{"name":"National research Institute of Rural Engineering Water and Forests University of Carthage  B.P. 10 Ariana 2080 Tunis Tunisia"}]},{"given":"Rita C.","family":"Alves","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV Department of Chemical Sciences Faculty of Pharmacy of the University of Porto  4050-313 Porto Portugal"}]},{"given":"Diana","family":"Melo","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV Department of Chemical Sciences Faculty of Pharmacy of the University of Porto  4050-313 Porto Portugal"}]},{"given":"Anabela S. G.","family":"Costa","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV Department of Chemical Sciences Faculty of Pharmacy of the University of Porto  4050-313 Porto Portugal"}]},{"given":"M. Antonia","family":"Nunes","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV Department of Chemical Sciences Faculty of Pharmacy of the University of Porto  4050-313 Porto Portugal"}]},{"given":"Abdelhamid","family":"Khaldi","sequence":"additional","affiliation":[{"name":"National research Institute of Rural Engineering Water and Forests University of Carthage  B.P. 10 Ariana 2080 Tunis Tunisia"}]},{"given":"M. Beatriz P. P.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV Department of Chemical Sciences Faculty of Pharmacy of the University of Porto  4050-313 Porto Portugal"}]},{"given":"Chokri","family":"Messaoud","sequence":"additional","affiliation":[{"name":"National Institute of Applied Science and Technology Department of Biology Laboratory of Nanobiotechnology and Valorization of Medicinal Phytoresources University of Carthage  B.P. 676 1080 Tunis Cedex Tunisia"}]}],"member":"311","published-online":{"date-parts":[[2021,5,4]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1177\/1082013206069910"},{"key":"e_1_2_7_2_1","article-title":"Seeds and seed oil compositions of Aleppo Pine (Pinus halepensis Mill.) grown in Jordan\u2019","author":"Tukan S. K.","year":"2013","journal-title":"Riv. Ital. Sostanze Grasse"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2013.12.051"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2018.10.038"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2012.01.016"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2007.07.012"},{"key":"e_1_2_7_7_1","first-page":"95","article-title":"Fatty acid and tocochromanol patterns of turkish pines\u2019","volume":"46","author":"Bagci E.","year":"2004","journal-title":"Acta Biol. Cracov. Ser. Bot."},{"key":"e_1_2_7_8_1","article-title":"Black Cumin (Nigella sativa L.) and Aleppo Pine (Pinus halepensis Mill.) Seed Oils: Stability During Thermal Oxidation at 60\u2009\u00b0C and 100\u2009\u00b0C\u2019","volume":"19","author":"Cheikh-Rouhou S.","year":"2008","journal-title":"Microbiol. Hyg. Alim."},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.5535"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7827(99)00008-9"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.021360"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/1082013208102695"},{"key":"e_1_2_7_13_1","first-page":"157","article-title":"Tocopherol content in edible plant oils\u2019","volume":"57","author":"Gliszczy\u0144ska-\u015awig\u0142o A.","year":"2007","journal-title":"Pol. J. Food Nutr. Sci."},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2007.09.001"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13197-012-0664-5"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/aje.12610"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp049568s"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1017571904517"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1051\/forest:20050100"},{"key":"e_1_2_7_20_1","unstructured":"I. NAHAL \u2018Taxonomie et aire g\u00e9ographique des pins du groupe halepensis\u2019 Options Mediterr.1986 1 1\u20139."},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:NEFO.0000022227.33131.f0"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10531-008-9390-8"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaridenv.2011.01.001"},{"key":"e_1_2_7_24_1","article-title":"Altitudinal variation of some morphological characters of Scots pine (Pinus sylvestris L.) in Turkey\u2019","volume":"8","author":"Turna I.","year":"2009","journal-title":"Afr. J. Biotechnol."},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2015.04.138"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13717-020-00264-3"},{"key":"e_1_2_7_27_1","first-page":"187","article-title":"Geographic cline and climatic effects on cone characteristics of natural populations of Pinus densiflora throughout the Japanese archipelago\u2019","volume":"24","author":"Iwaizumi M. G.","year":"2019","journal-title":"J. For. Res. (Philadelphia, PA, U.\u2005S."},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10342-019-01172-8"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202000117"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2020.112367"},{"key":"e_1_2_7_31_1","article-title":"Variations in oil, protein, fatty acids and vitamin E contents of pumpkin seeds under deficit irrigation\u2019","volume":"70","author":"Kirnak H.","year":"2019","journal-title":"Grasas Aceites (Sevilla, Spain"},{"key":"e_1_2_7_32_1","article-title":"Genotypic and Environmental Effects on Tocopherol Content in Almond\u2019","volume":"7","author":"Kodad O.","year":"2018","journal-title":"Antioxidants (Basel"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.7798"},{"key":"e_1_2_7_34_1","first-page":"443","article-title":"Exploring genetic variation, oil and \u03b1-tocopherol content in avocado (Persea americana) from northwestern Mexico\u2019","volume":"64","author":"Peraza-Magallanes A. Y.","year":"2017","journal-title":"Genet. Res."},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201700323"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2018.07.008"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11746-998-0008-9"},{"key":"e_1_2_7_38_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/PL00009698","article-title":"Taxonomy of Pinus species based on the seed oil fatty acid compositions\u2019","volume":"12","author":"Wolff R. L.","year":"1997","journal-title":"Trees Struct. Funct."},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/19476337.2016.1250109"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1021443719040101"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24112057"},{"key":"e_1_2_7_42_1","unstructured":"AOAC \u2018Official methods of analysis (19th ed.)\u2019 Arlington VA USA: Association of Official Analytical Chemists 2012."},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.03.106"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202100071","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202100071","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/cbdv.202100071","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202100071","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T20:02:46Z","timestamp":1693425766000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202100071"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,4]]},"references-count":43,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1002\/cbdv.202100071"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202100071","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"type":"print","value":"1612-1872"},{"type":"electronic","value":"1612-1880"}],"subject":[],"published":{"date-parts":[[2021,5,4]]},"assertion":[{"value":"2021-02-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2100071"}}