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In this study, we report the differences in the content of betulinic acid and selected anthraquinones in transgenic <jats:italic>S<\/jats:italic>.\u2005<jats:italic>obtusifolia<\/jats:italic> hairy roots with overexpression of the <jats:italic>PgSS1<\/jats:italic> gene (SOPSS2 line) and in transformed hairy roots without this genetic construct (SOA41 line). Both hairy root lines grew in 10\u2005L sprinkle bioreactor. Additionally, the extracts obtained from this plant material were used for biological tests. Our results demonstrated that the SOPSS2 hairy root cultures from the bioreactor showed an increase in the content of betulinic acid (38.125\u2005mg\/g DW), compared to the SOA41 hairy root line (4.213\u2005mg\/g DW). Biological studies have shown a cytotoxic and antiproliferative effect on U\u201087MG glioblastoma cells, and altering the level of apoptotic proteins (Bax, p53, Puma and Noxa). Antimicrobial properties were demonstrated for both tested extracts, with a stronger effect of SOPSS2 extract. Moreover, both extracts showed moderate antiviral properties on norovirus surrogates.<\/jats:p>","DOI":"10.1002\/cbdv.202100455","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T12:40:23Z","timestamp":1624970423000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Enhanced Accumulation of Betulinic Acid in Transgenic Hairy Roots of <i>Senna obtusifolia<\/i> Growing in the Sprinkle Bioreactor and Evaluation of Their Biological Properties in Various Biological Models"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4559-5015","authenticated-orcid":false,"given":"Tomasz","family":"Kowalczyk","sequence":"first","affiliation":[{"name":"Department of Molecular Biotechnology and Genetics University of Lodz  Banacha 12\/16 90-237 Lodz Poland"}]},{"given":"Przemys\u0142aw","family":"Sitarek","sequence":"additional","affiliation":[{"name":"Department of Biology and Pharmaceutical Botany Medical University of Lodz  Muszynskiego 1 \u0141\u00f3d\u017a 90-151 Lodz Poland"}]},{"given":"Monika","family":"Toma","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Genetics Faculty of Biology and Environmental Protection University of Lodz  Pomorska 141\/143 90-236 Lodz Poland"}]},{"given":"Patricia","family":"Rijo","sequence":"additional","affiliation":[{"name":"CBIOS \u2013 Research Center for Biosciences &amp; Health technologies Universidade Lus\u00f3fona de Humanidades e Tecnologias  Campo Grande 376 1749-024 Lisboa Portugal"},{"name":"iMed.ULisboa \u2013 Research Institute for Medicines Faculdade de Farm\u00e1cia da Universidade de Lisboa  Av. 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