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The DNA fragments were designed, cloned in Pet\u201021c expression vector and expressed in <jats:italic>E. coli<\/jats:italic> host as soluble protein. A solid\u2010phase combinatorial library based on the Ugi reaction was synthesized: 64 affinity ligands displaying complementary functionalities towards the designed tag. The library was screened by affinity chromatography in a 96\u2010well format for binding to the RKRKRK\u2010tagged GFP protein. Lead ligand A7C1 was selected for the purification of RKRKRK fusion proteins. The affinity pair RKRKRK\u2010tagged GFP with A7C1 emerged as a promising solution (<jats:italic>K<\/jats:italic><jats:sub>a<\/jats:sub> of 2.45\u00d710<jats:sup>5<\/jats:sup>\u2009<jats:sc>M<\/jats:sc><jats:sup>\u22121<\/jats:sup>). The specificity of the ligand towards the tag was observed experimentally and theoretically through automated docking and molecular dynamics simulations.<\/jats:p>","DOI":"10.1002\/cbic.201400018","type":"journal-article","created":{"date-parts":[[2014,6,5]],"date-time":"2014-06-05T15:51:30Z","timestamp":1401983490000},"page":"1423-1435","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A Tailor\u2010Made \u201cTag\u2013Receptor\u201d Affinity Pair for the Purification of Fusion Proteins"],"prefix":"10.1002","volume":"15","author":[{"given":"Ana S.","family":"Pina","sequence":"first","affiliation":[]},{"given":"M\u00e1rcia","family":"Guilherme","sequence":"additional","affiliation":[]},{"given":"Alice S.","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Cl\u00e1udia S. M.","family":"Fernandes","sequence":"additional","affiliation":[]},{"given":"Ricardo J. 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