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Here, we have developed an environmentally friendly two\u2010step process to transform the antibiotic rifampicin (RIF) into non\u2010antimicrobial compounds. The process involves an enzymatic oxidation step by the bacterial CotA\u2010laccase and a hydrogen peroxide bleaching step. NMR identified rifampicin quinone as the main product of the enzymatic oxidation. Growth of <jats:italic>Escherichia coli<\/jats:italic> strains in the presence of final degradation products (FP) and minimum inhibitory concentration (MIC) measurements confirmed that FP are non\u2010anti\u2010microbial compounds, and bioassays suggest that FP is not toxic to eukaryotic organisms. Moreover, competitive fitness assays between susceptible and RIF\u2010resistant bacteria show that susceptible bacteria is strongly favoured in the presence of FP. Our results show that we have developed a robust and environmentally friendly process to effectively remediate rifampicin from antibiotic contaminated environments.<\/jats:p>","DOI":"10.1002\/cbic.202300627","type":"journal-article","created":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T07:13:26Z","timestamp":1699600406000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Environmentally Friendly Degradation and Detoxification of Rifampicin by a Bacterial Laccase and Hydrogen Peroxide"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4411-2958","authenticated-orcid":false,"given":"Paulo","family":"Dur\u00e3o","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier Universidade Nova de Lisboa  Av da Rep\u00fablica 2780\u2013157 Oeiras Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8827-0635","authenticated-orcid":false,"given":"Peter","family":"Kis","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier Universidade Nova de Lisboa  Av da Rep\u00fablica 2780\u2013157 Oeiras Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5414-6625","authenticated-orcid":false,"given":"Ivo M.","family":"Chelo","sequence":"additional","affiliation":[{"name":"cE3c \u2013 Centre for Ecology Evolution and Environmental Changes &amp; CHANGE \u2013 Global Change and Sustainability Institute  1749-016 Lisboa Portugal"},{"name":"Departamento de Biologia Animal Faculdade de Ci\u00eancias Universidade de Lisboa  1749-016 Lisboa Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6854-7278","authenticated-orcid":false,"given":"M. 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