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During the biosynthetic reaction, hFAS incorporates acetyl and malonyl moieties through the action of its malonyl\u2010acetyl transferase (MAT) domain. These precursor molecules are transferred from CoA to the acyl\u2010carrier protein (ACP) domain by a two\u2010stage ping\u2010pong catalytic mechanism. The first stage, during which the acyl moieties are relocated from CoA to the MAT domain, has been elucidated in a previous work of ours. In this study, we employ QM\/MM calculations at the DLPNO\u2010CCSD(T)\/CBS:AMBER level of theory to understand the catalytic machinery behind the transfer of the acyl moieties from MAT to the ACP domain. The results show that the acyl transfer to the ACP's phosphopantetheine (PNS) occurs in two sequential events: Step 3) asynchronous concerted reaction that combines the deprotonation of the PNS's thiol group and the nucleophilic attack on the acyl\u2010Ser581 ester carbon; Step 4) tetrahedral intermediate breakdown and regeneration of the catalytic Ser581\/His683 dyad. The energy barriers of these two steps are inferior to those reported for the first catalytic stage, suggesting that the rate\u2010limiting step of the MAT mechanism lies on Stage 1, particularly on the concerted nucleophilic attack that starts the reaction. The oxyanion hole, formed by the backbone amines of Met499 and Leu582, was reported to transiently stabilize the tetrahedral intermediate and to play a favorable catalytic effect on the overall MAT reaction, particularly through the reduction of the energetic span. The knowledge gathered in this work complements previous experimental and theoretical studies and instigates further investigations that explore the therapeutic potential of hFAS.<\/jats:p>","DOI":"10.1002\/cctc.201900548","type":"journal-article","created":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T05:44:35Z","timestamp":1561614275000},"page":"3853-3864","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Human Fatty Acid Synthase: A Computational Study of the Transfer of the Acyl Moieties from MAT to the ACP Domain"],"prefix":"10.1002","volume":"11","author":[{"given":"Pedro","family":"Paiva","sequence":"first","affiliation":[{"name":"UCIBIO@REQUIMTE, Departamento de Qu\u00edmica e Bioqu\u00edmica Faculdade de Ci\u00eancias Universidade do Porto  Rua do Campo Alegre s\/n 4169-007 Porto Portugal"}]},{"given":"S\u00e9rgio F.","family":"Sousa","sequence":"additional","affiliation":[{"name":"UCIBIO@REQUIMTE, Departamento de Qu\u00edmica e Bioqu\u00edmica Faculdade de Ci\u00eancias Universidade do Porto  Rua do Campo Alegre s\/n 4169-007 Porto Portugal"},{"name":"UCIBIO@REQUIMTE, BioSIM \u2013 Departamento de Biomedicina Faculdade de Medicina Universidade do Porto Alameda Prof. Hern\u00e2ni Monteiro  4200-319 Porto Portugal"}]},{"given":"Pedro A.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"UCIBIO@REQUIMTE, Departamento de Qu\u00edmica e Bioqu\u00edmica Faculdade de Ci\u00eancias Universidade do Porto  Rua do Campo Alegre s\/n 4169-007 Porto Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7554-8324","authenticated-orcid":false,"given":"Maria","family":"Jo\u00e3o\u2005Ramos","sequence":"additional","affiliation":[{"name":"UCIBIO@REQUIMTE, Departamento de Qu\u00edmica e Bioqu\u00edmica Faculdade de Ci\u00eancias Universidade do Porto  Rua do Campo Alegre s\/n 4169-007 Porto Portugal"}]}],"member":"311","published-online":{"date-parts":[[2019,6,27]]},"reference":[{"key":"e_1_2_7_1_1","unstructured":"\u00a0"},{"key":"e_1_2_7_2_2","first-page":"18","volume":"39","author":"Calder P. 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