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However, the production cost of high\u2010quality IgY for large\u2010scale applications remains higher than that of other drug therapies due to the lack of efficient purification methods. The search for new purification platforms is thus vital. The solution could be liquid\u2013liquid extraction by using aqueous biphasic systems (ABS). Herein, we report the extraction and attempted purification of IgY from chicken egg yolk by using a new ABS composed of polymers and Good\u2019s buffer ionic liquids (GB\u2010ILs). New self\u2010buffering and biocompatible ILs based on the cholinium cation and anions derived from Good\u2019s buffers were synthesized and the self\u2010buffering characteristics and toxicity were characterized. Moreover, when these GB\u2010ILs are combined with PPG 400 (poly(propylene) glycol with a molecular weight of 400\u2005g\u2009mol<jats:sup>\u20101<\/jats:sup>) to form ABS, extraction efficiencies, of the water\u2010soluble fraction of proteins, ranging between 79 and 94\u2009% were achieved in a single step. Based on computational investigations, we also demonstrate that the preferential partitioning of IgY for the GB\u2010IL\u2010rich phase is dominated by hydrogen\u2010bonding and van der Waals interactions.<\/jats:p>","DOI":"10.1002\/chem.201405693","type":"journal-article","created":{"date-parts":[[2015,2,4]],"date-time":"2015-02-04T15:07:09Z","timestamp":1423062429000},"page":"4781-4788","source":"Crossref","is-referenced-by-count":103,"title":["Novel Biocompatible and Self\u2010buffering Ionic Liquids for Biopharmaceutical Applications"],"prefix":"10.1002","volume":"21","author":[{"given":"Mohamed","family":"Taha","sequence":"first","affiliation":[]},{"given":"Mafalda R.","family":"Almeida","sequence":"additional","affiliation":[]},{"given":"Francisca A. e.","family":"Silva","sequence":"additional","affiliation":[]},{"given":"Pedro","family":"Domingues","sequence":"additional","affiliation":[]},{"given":"S\u00f3nia P. 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