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Since we previously found that the number of granule cells of the dentate gyrus is reduced in hypothyroid animals, we decided to extend our observations to the pyramidal cells of the hippocampus in order to gain further insight into the effects of hypothyroidism upon the other neuronal links of the hippocampal trisynaptic circuitry, inasmuch as CA1 neurons are known to be particularly vulnerable to aggressive agents.<\/jats:p><jats:p>Groups of 6 male and 6 female rats aged 30 and 180 days were analysed separately after being treated as follows: (1) hypothyroid from day 0 until day 30 (30\u2010day\u2010old hypothyroid group); (2) respective 30\u2010day\u2010old control; (3) hypothyroid from day 0 until day 180 (180\u2010day\u2010old hypothyroid group); (4) hypothyroid until day 30 and thenceforth maintained euthyroid (recovery group); (5) hypothyroid since day 30 (adult hypothyroid group); and (6) respective 180\u2010day\u2010old control. The volume of the pyramidal cell layer of the CA1 and CA3 regions and the numerical density of the respective neurons were evaluated, thereby allowing us to estimate the total number of pyramidal cells in each hippocampal region. The areal density and the mean nuclear volume of CA1 and CA3 pyramidal cells were also estimated.<\/jats:p><jats:p>In the CA3 region, we found that hypothyroidism, whatever its duration and time of onset, induces a reduction in the volume of the pyramidal cell layer and a parallel increase in the numerical density of its neurons, without interfering with the total number of pyramidal cells. Conversely, in the CA1 region, thyroid hormone deficiency started either neonatally or during maturity was found to lead to a decrease in the total number of pyramidal cells. Reductions ranging between 14.2 and 22.5% were found in 30 and 180\u2010day\u2010old hypothyroid groups. The reestablishment of a euthyroid state did not ameliorate the referred neuronal loss.<\/jats:p><jats:p>The present results support the view that hypothyroidism induces small alterations in the structural organization of the hippocampal CA3 region, contrary to what happens in CA1 in which neuronal death occurs. Furthermore, the data presented herein demonstrate that the total number of CA1 pyramidal cells displays sexual dimorphism that is not affected by thyroid hormone manipulations. These findings allow us to admit that hypothyroidism induces anatomical changes in the hippocampus which are in line with those reported for the dentate gyrus, thereby providing additional morphological basis for the cognitive alterations described in this condition. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.903220405","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T21:43:49Z","timestamp":1117230229000},"page":"501-518","source":"Crossref","is-referenced-by-count":111,"title":["Selective vulnerability of the hippocampal pyramidal neurons to hypothyroidism in male and female rats"],"prefix":"10.1002","volume":"322","author":[{"given":"M. D.","family":"Madeira","sequence":"first","affiliation":[]},{"given":"N.","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"M. 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