{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:26:11Z","timestamp":1761611171481,"version":"build-2065373602"},"reference-count":38,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,11,14]],"date-time":"2005-11-14T00:00:00Z","timestamp":1131926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemPhysChem"],"published-print":{"date-parts":[[2005,12,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>The adsorption of amyloid<\/jats:italic> \u03b2<jats:italic>\u2010peptide at hydrophilic and hydrophobic modified silicon\u2013liquid interfaces was characterized by neutron reflectometry. Distinct polymeric films were used to obtain noncharged (Formvar), negatively (sodium poly(styrene sulfonate)) and positively charged (poly(allylamine hydrochloride)) hydrophilic as well as hydrophobic surfaces (polystyrene and a polysiloxane\u2013dodecanoic acid complex). Amyloid<\/jats:italic> \u03b2<jats:italic>\u2010peptide was found to adsorb at positively charged hydrophilic and hydrophobic surfaces, whereas no adsorbed layer was detected on hydrophilic noncharged and negatively charged films. The peptide adsorbed at the positively charged film as patches, which were dispersed on the surface, whereas a uniform layer was observed at hydrophobic surfaces. The thickness of the adsorbed peptide layer was estimated to be approximately 20 \u00c5. The peptide formed a tightly packed layer, which did not contain water. These studies provide information about the affinity of the amyloid<\/jats:italic> \u03b2<jats:italic>\u2010peptide to different substrates in aqueous solution and suggest that the amyloid fibril formation may be driven by interactions with surfaces.<\/jats:italic><\/jats:p>","DOI":"10.1002\/cphc.200500158","type":"journal-article","created":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T18:19:03Z","timestamp":1132165143000},"page":"2527-2534","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Adsorption of Amyloid \u03b2\u2010Peptide at Polymer Surfaces: A Neutron Reflectivity Study"],"prefix":"10.1002","volume":"6","author":[{"given":"Sandra","family":"Rocha","sequence":"first","affiliation":[]},{"given":"Rumen","family":"Krastev","sequence":"additional","affiliation":[]},{"given":"Andreas F.","family":"Th\u00fcnemann","sequence":"additional","affiliation":[]},{"given":"M. Carmo","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Helmuth","family":"M\u00f6hwald","sequence":"additional","affiliation":[]},{"given":"Gerald","family":"Brezesinski","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,12,6]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02264"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.3342"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb05554.x"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.2.503"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/325733a0"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/359322a0"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/359325a0"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1853202"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(91)90881-6"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.51.32916"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0940"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1095-1062"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.3688"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)77161-4"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/cphc.200400045"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200500116"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5330.1232"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/la990650k"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/ma0351930"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ma971196z"},{"volume-title":"Modern characterization methods of surfactant systems, Surfactant science series 83","year":"1999","author":"Thomas R. K.","key":"e_1_2_7_21_2"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1088\/0953-8984\/13\/21\/322"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/la0486436"},{"key":"e_1_2_7_24_2","unstructured":"C. Braun Parratt32 Fitting routine for reflectivity data HMI Berlin 1997\u20131999."},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.250288897"},{"key":"e_1_2_7_26_2","first-page":"141","volume-title":"Protein: Structure and Molecular properties","author":"Creighton T. E.","year":"1992"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/10448639208218770"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0920-2307(05)80002-7"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0927-7765(94)80066-9"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9797(88)90001-X"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1116\/1.585208"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja990864o"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.2000.4288"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00483a001"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0292290"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0921-4526(95)00317-3"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRev.95.359"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1021\/j100830a521"}],"container-title":["ChemPhysChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcphc.200500158","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cphc.200500158","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T08:08:37Z","timestamp":1760342917000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cphc.200500158"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,12,6]]},"references-count":38,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2005,12,9]]}},"alternative-id":["10.1002\/cphc.200500158"],"URL":"https:\/\/doi.org\/10.1002\/cphc.200500158","archive":["Portico"],"relation":{},"ISSN":["1439-4235","1439-7641"],"issn-type":[{"type":"print","value":"1439-4235"},{"type":"electronic","value":"1439-7641"}],"subject":[],"published":{"date-parts":[[2005,12,6]]},"assertion":[{"value":"2005-03-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-12-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}