{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T18:15:24Z","timestamp":1760897724544,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemPhysChem"],"published-print":{"date-parts":[[2006,8,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The excited\u2010state processes of protochlorophyllide a, the precursor of chlorophyll a in chlorophyll biosynthesis, are studied using picosecond time\u2010resolved fluorescence spectroscopy. Following excitation into the Soret band, two distinct fluorescence components, with emission maxima at 640 and 647 nm, are observed. The 640 nm emitting component appears within the time resolution of the experiment and then decays with a time constant of 27 ps. In contrast, the 647 nm emitting component is built up with a 3.5 ps rise time and undergoes a subsequent decay with a time constant of 3.5 ns. The 3.5 ps rise kinetics are attributed to relaxations in the electronically excited state preceding the nanosecond fluorescence, which is ascribed to emission out of the thermally equilibrated S<jats:sub>1<\/jats:sub> state. The 27 ps fluorescence, which appears within the experimental response of the streak camera, is suggested to originate from a second minimum on the excited\u2010state potential\u2010energy surface. The population of the secondary excited state is suggested to reflect a very fast motion out of the Franck\u2013Condon region along a reaction coordinate different from the one connecting the Franck\u2013Condon region with the S<jats:sub>1<\/jats:sub> potential\u2010energy minimum. The 27 ps\u2010component is an emissive intermediate on the reactive excited\u2010state pathway, as its decay yields the intermediate photoproduct, which has been identified previously (J. Phys. Chem. B <jats:bold>2006<\/jats:bold>, <jats:italic>110<\/jats:italic>, 4399\u20134406). No emission of the photoproduct is observed. The results of the time\u2010resolved fluorescence study allow a detailed spectral characterization of the emission of the excited states in protochlorophyllide a, and the refinement of the kinetic model deduced from ultrafast absorption measurements.<\/jats:p>","DOI":"10.1002\/cphc.200600172","type":"journal-article","created":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T07:25:35Z","timestamp":1152861935000},"page":"1727-1733","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["The Excited\u2010State Chemistry of Protochlorophyllide a: A Time\u2010Resolved Fluorescence Study"],"prefix":"10.1002","volume":"7","author":[{"given":"Benjamin","family":"Dietzek","sequence":"first","affiliation":[]},{"given":"Wolfgang","family":"Kiefer","sequence":"additional","affiliation":[]},{"given":"Arkady","family":"Yartsev","sequence":"additional","affiliation":[]},{"given":"Villy","family":"Sundstr\u00f6m","sequence":"additional","affiliation":[]},{"given":"Peter","family":"Schellenberg","sequence":"additional","affiliation":[]},{"given":"Paulius","family":"Grigaravicius","sequence":"additional","affiliation":[]},{"given":"Gudrun","family":"Hermann","sequence":"additional","affiliation":[]},{"given":"J\u00fcrgen","family":"Popp","sequence":"additional","affiliation":[]},{"given":"Michael","family":"Schmitt","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,8,3]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.31.1.61"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006297731456"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(03)00064-5"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-004-2331-3"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0330737"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1950083"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.9.3749"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.3.724"},{"key":"e_1_2_7_9_2","first-page":"13","volume-title":"The Porphyrin Handbook","author":"R\u00fcdiger W.","year":"2003"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1562\/0031-8655(2003)0780543PRMAE2.0.CO2"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:PRES.0000028392.80354.7c"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2004-821270"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsb929"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp0556456"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cplett.2004.08.075"},{"key":"e_1_2_7_16_2","first-page":"1","volume-title":"The Porphyrins","author":"Gouterman M.","year":"1978"},{"key":"e_1_2_7_17_2","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1111\/j.1751-1097.2004.tb09858.x","volume":"79","author":"Mysliwa\u2010Kurdziel B.","year":"2004","journal-title":"Photochem. Photobiol."},{"key":"e_1_2_7_18_2","first-page":"42","author":"Elsaesser T.","year":"1991","journal-title":"Annu. Rev. Phys. Chem."},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/j100048a004"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp0203999"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp020398g"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cplett.2005.08.136"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr020674n"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.182274199"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi049576h"},{"key":"e_1_2_7_26_2","first-page":"74","author":"Raskin V. I.","year":"2002","journal-title":"Photosynth. Res."},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2005.09.001"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00627.x"},{"key":"e_1_2_7_29_2","unstructured":"The absolute wavelength numbers of the fluorescence given in the paper correspond to the calibration and correction curves as established for the various experimental setups (steady\u2010state fluorescence spectrometer and the two different streak camera setups). These experiments were not cross\u2010calibrated and calibrations and corrections might have shifted over time. Thus slight differences in absolute values for the emission maxima as obtained from various experiments might be attributed to these experimental uncertainties."}],"container-title":["ChemPhysChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcphc.200600172","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cphc.200600172","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T20:41:26Z","timestamp":1760388086000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cphc.200600172"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8,3]]},"references-count":29,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2006,8,11]]}},"alternative-id":["10.1002\/cphc.200600172"],"URL":"https:\/\/doi.org\/10.1002\/cphc.200600172","archive":["Portico"],"relation":{},"ISSN":["1439-4235","1439-7641"],"issn-type":[{"type":"print","value":"1439-4235"},{"type":"electronic","value":"1439-7641"}],"subject":[],"published":{"date-parts":[[2006,8,3]]},"assertion":[{"value":"2006-03-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-08-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}