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Hybridization efficiencies of about 70\u2009% are obtained between the DNA immobilized in the particles and a complementary strand in solution, even at very low ionic strengths (1\u2009m<jats:sc>M<\/jats:sc>). The polymer nanoparticles have a glassy poly(methylmethacrylate) (PMMA) core and a thermoresponsive shell of poly(<\/jats:italic>N<jats:italic>\u2010isopropylacrylamide) (PNIPAM) containing positive charges. After a DNA strand labeled with a fluorescence probe is loaded onto the particles at room temperature, the temperature is increased above the volume phase transition temperature of the PNIPAM shell, TVPT \u224828\u2009\u00b0C. The collapse of the particle shell immobilizes the DNA while maintaining its availability for hybridization with a complementary strand. F\u00f6rster resonance energy transfer (FRET) is used to detect the hybridization with a complementary DNA strand labeled with a FRET acceptor probe.<\/jats:italic><\/jats:p>","DOI":"10.1002\/cphc.201000015","type":"journal-article","created":{"date-parts":[[2010,4,15]],"date-time":"2010-04-15T15:07:53Z","timestamp":1271344073000},"page":"1749-1756","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["DNA Hybridization in Thermoresponsive Polymer Nanoparticles"],"prefix":"10.1002","volume":"11","author":[{"given":"Leila M.","family":"Moura","sequence":"first","affiliation":[]},{"given":"Jose M. 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