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In this work, a central composite design (CCD) of experiments and response surface methodology (RSM) were used to study, for the first time, the L\u2010asparaginase (ASNase) immobilization onto functionalized carbon xerogels (CXs). The best results were achieved using CXs obtained by hydrothermal oxidation with nitric acid and subsequent heat treatment in a nitrogen flow at 600\u2009\u00b0C (CX\u2212OX\u2010600). Under the optimal conditions (81\u2005min of contact time, pH\u20056.2 and 0.36\u2005g\/L of ASNase), an immobilization yield (<jats:italic>IY<\/jats:italic>) of 100\u2009% and relative recovered activity (<jats:italic>RRA<\/jats:italic>) of 103\u2009% were achieved. The kinetic parameters obtained also indicate a 1.25\u2010fold increase in the affinity of ASNase towards the substrate after immobilization. Moreover, the immobilized enzyme retained 97\u2009% of its initial activity after 6 consecutive reaction cycles. All these outcomes confirm the promising properties of functionalized CXs as support for ASNase, bringing new insights into the development of an efficient and stable immobilization platform for use in the pharmaceutical industry, food industry, and biosensors.<\/jats:p>","DOI":"10.1002\/cplu.202400025","type":"journal-article","created":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T11:29:46Z","timestamp":1709551786000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Improved L\u2010Asparaginase Properties and Reusability by Immobilization onto Functionalized Carbon Xerogels"],"prefix":"10.1002","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5627-0785","authenticated-orcid":false,"given":"Rita A. 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