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The analysis of the photophysical properties revealed that the increase in the number of DMAP units on ZnPc shifted the absorption and emission band in aqueous media to the red region. The presence of multi\u2010positive charges on ZnPc derivatives prevented aggregation and enhances the solubility in aqueous media. The quaternized derivatives ZnPc <jats:bold>1\u2009a<\/jats:bold> and <jats:bold>2\u2009a<\/jats:bold> displayed good stability and promising efficacy to generate singlet oxygen (<jats:sup>1<\/jats:sup>O<jats:sub>2<\/jats:sub>). The affinity of the amphiphilic ZnPc <jats:bold>1<\/jats:bold>\u2013<jats:bold>2<\/jats:bold> and quaternized ZnPc <jats:bold>1\u2009a<\/jats:bold>\u2013<jats:bold>2\u2009a<\/jats:bold> to planktonic bacterial cells corresponded to an average uptake of \u223c10<jats:sup>6<\/jats:sup>\u2005PS molecules.CFU<jats:sup>\u22121<\/jats:sup>. The PDI assays conducted with planktonic cells and biofilms of <jats:italic>E. coli<\/jats:italic> show that irradiation with red or white light (150\u2005mW.cm<jats:sup>\u22122<\/jats:sup>) in the presence of 20\u2005\u03bcM of ZnPc derivatives caused an effective inactivation. ZnPc <jats:bold>1\u2009a<\/jats:bold> and ZnPc <jats:bold>2\u2009a<\/jats:bold> exhibited the highest inactivation efficiency, particularly of the planktonic form, causing a 5\u2005log\u2010scale (99.99\u2009%) reduction in bioluminescence. The inactivation factor for biofilms was 99\u2009% (2\u2005log). ZnPc <jats:bold>1\u2009a<\/jats:bold> and ZnPc <jats:bold>2\u2009a<\/jats:bold> can be regarded as promising photosensitizers for the photodynamic inactivation of Gram\u2010negative bacteria.<\/jats:p>","DOI":"10.1002\/cptc.201900020","type":"journal-article","created":{"date-parts":[[2019,1,31]],"date-time":"2019-01-31T01:20:37Z","timestamp":1548897637000},"page":"251-260","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Photoinactivation of Planktonic and Biofilm Forms of <i>Escherichia coli<\/i> through the Action of Cationic Zinc(II) Phthalocyanines"],"prefix":"10.1002","volume":"3","author":[{"given":"Leandro M. O.","family":"Louren\u00e7o","sequence":"first","affiliation":[{"name":"QOPNA-LAQV-REQUINTE and Department of Chemistry University of Aveiro  Campus Universit\u00e1rio de Santiago 3810-193 Aveiro Portugal"}]},{"given":"Deisy M. G. 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V.","family":"Ramos","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Laboratory and Department of Chemistry University of Aveiro  Campus Universit\u00e1rio de Santiago 3810-193 Aveiro Portugal"}]},{"given":"Maria C.","family":"Gomes","sequence":"additional","affiliation":[{"name":"QOPNA-LAQV-REQUINTE and Department of Chemistry University of Aveiro  Campus Universit\u00e1rio de Santiago 3810-193 Aveiro Portugal"},{"name":"CESAM and Department of Biology University of Aveiro  Campus Universit\u00e1rio de Santiago 3810-193 Aveiro Portugal"}]},{"given":"Adelaide","family":"Almeida","sequence":"additional","affiliation":[{"name":"CESAM and Department of Biology University of Aveiro  Campus Universit\u00e1rio de Santiago 3810-193 Aveiro Portugal"}]},{"given":"Maria A. F.","family":"Faustino","sequence":"additional","affiliation":[{"name":"QOPNA-LAQV-REQUINTE and Department of Chemistry University of Aveiro  Campus Universit\u00e1rio de Santiago 3810-193 Aveiro Portugal"}]},{"given":"Filipe A.","family":"Almeida\u2005Paz","sequence":"additional","affiliation":[{"name":"CICECO-Aveiro Institute of Materials and Department of Chemistry University of Aveiro  Campus Universit\u00e1rio de Santiago 3810-193 Aveiro Portugal"}]},{"given":"Maria G. P. M. S.","family":"Neves","sequence":"additional","affiliation":[{"name":"QOPNA-LAQV-REQUINTE and Department of Chemistry University of Aveiro  Campus Universit\u00e1rio de Santiago 3810-193 Aveiro Portugal"}]},{"given":"\u00c2ngela","family":"Cunha","sequence":"additional","affiliation":[{"name":"CESAM and Department of Biology University of Aveiro  Campus Universit\u00e1rio de Santiago 3810-193 Aveiro Portugal"}]},{"given":"Jo\u00e3o P. 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