{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:13:09Z","timestamp":1760548389533},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,12]],"date-time":"2006-07-12T00:00:00Z","timestamp":1152662400000},"content-version":"vor","delay-in-days":3906,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[1995,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We describe a method of immunocytochemically assessing estrogen receptor (ER) status on alcohol\u2010fixed smears obtained by fine\u2010needle aspiration (FNA) from breast cancer patients, using a commercially available monoclonal antibody (1D5) with microwave oven processing. A series of 31 cases of aspirates from breast cancer were analysed and the results were compared with assessment by ER immunocytochemical assay using the same procedure on formalin\u2010fixed tissue and with assessment by ER\u2010ICA assay on frozen sections. The results were scored semiquantitatively using a five grade scoring system. Of the 31 cases examined, 21 were positive at least by two methods and 10 were negative for all three determinations. The results obtained in the ER immunocytochemical assay on aspirates and paraffin\u2010sections using the antibody 1D5 and those obtained on frozen sections using the antibody H222 were closely similar. In only one case was it not possible to interpret the reaction in the cytological specimen because there was a strong background in the smear. In general, we obtained more intense positivity with the antibody 1D5 in aspirates and formalin\u2010fixed material than with the antibody H222 in frozen sections. The scoring results of the three methods were almost identical. We conclude that the application of ER method on alcohol\u2010fixed smears will eliminate the need for using a special fixation procedure and will provide several advantages, such as: improvement in morphological concomitant analysis, utilization whenever malignancy is found without necessity to re\u2010aspirate the patient, and adequacy of archival material. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dc.2840130417","type":"journal-article","created":{"date-parts":[[2007,2,22]],"date-time":"2007-02-22T05:15:51Z","timestamp":1172121351000},"page":"347-351","source":"Crossref","is-referenced-by-count":33,"title":["Estimation of estrogen receptor content in fine\u2010needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations"],"prefix":"10.1002","volume":"13","author":[{"given":"Fernando Carlos","family":"Schmitt","sequence":"first","affiliation":[]},{"given":"Maria Jos\u00e9","family":"Bento","sequence":"additional","affiliation":[]},{"given":"Isabel","family":"Amendoeira","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,7,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1001\/jama.1991.03460030097037","article-title":"NIH consensus conference: treatment of early stage breast cancer","volume":"265","author":"National Institutes of Health","year":"1991","journal-title":"JAMA"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.2840080402"},{"key":"e_1_2_1_4_2","first-page":"282","article-title":"Estrogen and progesterone receptors and modal DNA value in tumor cells obtained by fine\u2010needle aspiration from primary breast carcinomas during tamoxifen treatment","volume":"1","author":"Skoog L","year":"1991","journal-title":"Diagn Oncol"},{"key":"e_1_2_1_5_2","first-page":"6126","article-title":"Prognostic value of steroid hormone receptors: multivariate analysis of systemically untreated patients with node negative primary breast cancer","volume":"47","author":"Thorpe SM","year":"1987","journal-title":"Cancer Res"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.2840080508"},{"key":"e_1_2_1_7_2","first-page":"26","article-title":"Use of monoclonal antibody for assessment of estrogen receptor content in fine\u2010needle aspiration biopsy specimen from patients with breast cancer","volume":"113","author":"Masood S","year":"1989","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.47.1.90"},{"key":"e_1_2_1_9_2","volume-title":"Monographs of clinical cytology","author":"Zacijek J","year":"1974"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.9.5115"},{"key":"e_1_2_1_11_2","first-page":"275","article-title":"Immunohistochemical analysis of breast carcinoma estrogen and progesterone receptors in paraffin\u2010embedded tissue. Correlation of clones ER1D5 and 1A6 with a cytosol\u2010based hormone assay","volume":"1","author":"Kell DL","year":"1993","journal-title":"Appl Immunohistochem"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(11)81095-2"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1993.tb01195.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1992.tb00442.x"},{"key":"e_1_2_1_15_2","first-page":"158","article-title":"Detection of estrogen receptor in formalin\u2010fixed and paraffin\u2010embedded breast carcinoma: correlation with histological patterns","volume":"110","author":"Schmitt FC","year":"1992","journal-title":"Rev Paul Med"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdc.2840130417","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.2840130417","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T06:42:41Z","timestamp":1698302561000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.2840130417"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,11]]},"references-count":14,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1995,11]]}},"alternative-id":["10.1002\/dc.2840130417"],"URL":"https:\/\/doi.org\/10.1002\/dc.2840130417","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,11]]}}}