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However, no complete mitochondrial genome has been reported for this genus, leaving its mitogenomic characteristics and evolutionary patterns unclear. In this study, we sequenced and characterized the first complete mitogenome of\n                    <jats:italic>Acytolepis puspa<\/jats:italic>\n                    , the type species of the genus. The circular double\u2010stranded mitogenome is 15,511\u2009bp in length and comprises 13 protein\u2010coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and one A\u2009+\u2009T\u2010rich control region, exhibiting a typical gene content and organization conserved in Lepidoptera. Comparative analyses of Polyommatinae mitogenomes revealed pronounced heterogeneity in evolutionary rates among PCGs, with\n                    <jats:italic>ND6<\/jats:italic>\n                    and\n                    <jats:italic>ND3<\/jats:italic>\n                    showing relatively high nucleotide diversity, whereas\n                    <jats:italic>COX1<\/jats:italic>\n                    was the most conserved gene. Selection pressure analyses indicated that all PCGs are evolving under purifying selection, with\n                    <jats:italic>ATP8<\/jats:italic>\n                    and\n                    <jats:italic>ND6<\/jats:italic>\n                    exhibiting relatively relaxed selective constraints compared to other genes. Phylogenetic analyses based on concatenated mitochondrial PCGs using both Maximum likelihood (ML) and Bayesian inference (BI) methods produced identical and well\u2010supported topologies, recovering\n                    <jats:italic>A. puspa<\/jats:italic>\n                    as the sister taxon to\n                    <jats:italic>Celastrina<\/jats:italic>\n                    species within Polyommatinae. Overall, this study provides the first mitogenomic resource for\n                    <jats:italic>Acytolepis<\/jats:italic>\n                    , enriches mitochondrial molecular markers for lycaenid butterflies, and contributes new insights into mitogenome evolution and phylogenetic relationships within Polyommatinae.\n                  <\/jats:p>","DOI":"10.1002\/ece3.73326","type":"journal-article","created":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T23:19:54Z","timestamp":1774826394000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The First Complete Mitochondrial Genome of Common Hedge Blue\n                    <i>Acytolepis puspa<\/i>\n                    (Lepidoptera: Lycaenidae), and Comparative Genomic Analysis Within Polyommatinae"],"prefix":"10.1002","volume":"16","author":[{"given":"Muyang","family":"Li","sequence":"first","affiliation":[{"name":"School of Life Sciences Qufu Normal 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