{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:16:48Z","timestamp":1770718608941,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,12,11]],"date-time":"2005-12-11T00:00:00Z","timestamp":1134259200000},"content-version":"vor","delay-in-days":4484,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1993,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In T lymphocytes, CD2 forms part of a loosely associated membrane complex which includes the T cell receptor (TcR) for antigen, the CD3 subunits, CD4 or CD8, CD5 and the protein tyrosine kinases p56<jats:sup><jats:italic>lck<\/jats:italic><\/jats:sup> and p59<jats:sup><jats:italic>fyn<\/jats:italic><\/jats:sup>. The interaction of CD2 with tyrosine kinases in this complex provides a possible mechanism for transmembrane signal transduction by CD2. We have investigated whether the interaction of CD2 with the kinases is dependent on other known members of the complex, or whether an independent association can be observed. Using <jats:italic>in vitro<\/jats:italic> kinase assays with immune complexes precipitated from cell lysates, we demonstrate that CD2 can associate with p56<jats:sup><jats:italic>lck<\/jats:italic><\/jats:sup> and p59<jats:sup><jats:italic>fyn<\/jats:italic><\/jats:sup> in a rat thymoma line that does not express CD4 or CD8, and in a TcR\u2010negative Jurkat cell line. In TcR\u2010positive Jurkat cells that express rat CD2, interaction of CD2 with p56<jats:sup><jats:italic>lck<\/jats:italic><\/jats:sup> and p59<jats:sup><jats:italic>fyn<\/jats:italic><\/jats:sup> <jats:sub>W<\/jats:sub>as clearly seen, but it was absent in cells where the cytoplasmic tail of CD2 is truncated, indicating that the interactions are mediated by the cytoplasmic region of CD2. Furthermore, using cells expressing CD2 molecules with partial truncations in the cytoplasmic domain, we show that the association of CD2 with p56<jats:italic><jats:sup>lck:<\/jats:sup><\/jats:italic> is progressively lost as the cytoplasmic domain is shortened, and that the capacity of the mutants to associate with p56<jats:sup><jats:italic>lck<\/jats:italic><\/jats:sup> correlates with their capacity to transduce transmembrane signals.<\/jats:p>","DOI":"10.1002\/eji.1830230922","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T19:24:01Z","timestamp":1172777041000},"page":"2196-2201","source":"Crossref","is-referenced-by-count":60,"title":["Physical association of the cytoplasmic domain of CD2 with the tyrosine kinases p56<sup>lck<\/sup> and p59<sup>fyn<\/sup>"],"prefix":"10.1002","volume":"23","author":[{"given":"Alexandre M.","family":"Carmo","sequence":"first","affiliation":[]},{"given":"Don W.","family":"Mason","sequence":"additional","affiliation":[]},{"given":"Albertus D.","family":"Beyers","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,12,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/360232a0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.10.3365"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.165.2.368"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830170718"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90039-4"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.6.1861"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.3.1145"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90112-2"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830210828"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.7.2945"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90090-2"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.12.12.5438"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/298170a0"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.133.1.123"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1989.tb00542.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"3660","DOI":"10.4049\/jimmunol.139.11.3660","volume":"139","author":"Waldor M. 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