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By immunoprecipitation from detergent lysates and <jats:italic>in vitro<\/jats:italic> immune complex kinase reactions the GPI\u2010anchored molecules Thy\u20101 and CD48 are shown to be associated with multimolecular complexes of phosphoproteins including the protein tyrosine kinases p56<jats:italic><jats:sup>lck:<\/jats:sup><\/jats:italic> and p60<jats:italic><jats:sup>fyn<\/jats:sup><\/jats:italic> in both rat and mouse thymocytes. Moreover, the kinase activity associated with Thy\u20101 on rat thymocytes is shown to be dependent on the activation state of the cells, with stimulation by the lectin, concanavalin A, producing a marked decrease in Thy\u20101\u2010associated kinase activity. In such activated cells, there is an increased association of kinase activity with CD48, but this may be explained in terms of increased surface expression of CD48 and of increased total kinase activity. Additional phosphoproteins of 85, 36 and 32 kDa were consistently seen as components of the complexes.<\/jats:p>","DOI":"10.1002\/eji.1830231024","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T18:36:00Z","timestamp":1172774160000},"page":"2540-2544","source":"Crossref","is-referenced-by-count":40,"title":["The association of the protein tyrosine kinases p56<sup><i>lck<\/i><\/sup> and p60<sup><i>fyn<\/i><\/sup> with the glycosyl phosphatidylinositol\u2010anchored proteins Thy\u20101 and CD48 in rat thymocytes is dependent on the state of cellular activation"],"prefix":"10.1002","volume":"23","author":[{"given":"David","family":"Garnett","sequence":"first","affiliation":[]},{"given":"A.","family":"Neil Barclay","sequence":"additional","affiliation":[]},{"given":"Alexandre M.","family":"Carmo","sequence":"additional","affiliation":[]},{"given":"Albertus D.","family":"Beyers","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.57.070188.001441"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.06.040188.002121"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.2.515"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"4379","DOI":"10.4049\/jimmunol.136.12.4379","volume":"136","author":"Kroczek R.","year":"1986","journal-title":"J. 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